Record Details

Effects of lyotropic anions on thermodynamic stability and dynamics of horse cytochrome c.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Effects of lyotropic anions on thermodynamic stability and dynamics of horse cytochrome c.
 
Creator Jain, Rishu
Agarwal, Mukesh Chand
Kumar, Rakesh
Sharma, Deepak
Kumar, Rajesh
 
Subject QR Microbiology
 
Description This paper evaluates the effect of various lyotropic anions (chloride, sulfate, perchlorate, iodide, nitrate, bromide) on the thermodynamic stability and dynamics of native cytochrome c (Cyt c) at pH 7.0. The results of equilibrium and kinetic studies revealed that: (i) at low to intermediate concentrations (≤ 0.5 M), both chaotropic and kosmotropic anions restrict the dynamics of native protein, (ii) at relatively higher concentrations (≥ 1.0 M), the denaturing effect of chaotropic anions dominates, which increases the level of structural-fluctuations responsible to unfold the protein according to Hofmeister series (perchlorate > iodide > nitrate > bromide), and (iii) the lyotropic anions affect the thermal and global stability of Cyt c according to Hofmeister series. The m-value was determined from ΔΔG vs [Cosolute] plot and was found to be positive for sulfate and negative for other anions consistent with effect of lyotopic anions on protein stability according to Hofmeister series.
 
Publisher Elsevier Science Ltd
 
Date 2018-06-19
 
Type Article
PeerReviewed
 
Relation https://www.sciencedirect.com/science/article/abs/pii/S0301462218300875
http://crdd.osdd.net/open/2092/
 
Identifier Jain, Rishu and Agarwal, Mukesh Chand and Kumar, Rakesh and Sharma, Deepak and Kumar, Rajesh (2018) Effects of lyotropic anions on thermodynamic stability and dynamics of horse cytochrome c. Biophysical chemistry, 240. pp. 88-97. ISSN 1873-4200