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Acidovorax kalamii sp. nov., isolated from a water sample of the river Ganges.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Acidovorax kalamii sp. nov., isolated from a water sample of the river Ganges.
 
Creator Pal, Deepika
Kaur, Navjot
Sudan, Sarabjeet Kour
Bisht, Bhawana
Krishnamurthi, Srinivasan
Mayilraj, Shanmugam
 
Subject QR Microbiology
 
Description A Gram-stain-negative, rod-shaped, aerobic, straw yellow, motile strain, designated KNDSW-TSA6, belonging to the genus Acidovorax, was isolated from a water sample of the river Ganges, downstream of the city of Kanpur, Uttar Pradesh, India. Cells were aerobic, non-endospore-forming and motile with single polar flagella. It differed from its phylogenetically related strains by phenotypic characteristics such as hydrolysis of urea, gelatin, casein and DNA, and the catalase reaction. The major fatty acids were C16 : 1ω7c/C16 : 1ω6c, C16 : 0 and C18 : 1ω7c/C18 : 1ω6c. Phylogenetic analysis based on 16S rRNA and housekeeping genes (gyrb, recA and rpoB gene sequences), confirmed its placement within the genus Acidovorax as a novel species. Strain KNDSW-TSA6 showed highest 16S rRNA sequence similarity to Acidovorax soli BL21 (98.9 %), Acidovorax delafieldii ATCC 17505 (98.8 %), Acidovorax temperans CCUG 11779 (98.2 %), Acidovorax caeni R-24608 (97.9 %) and Acidovorax radicis N35 (97.6 %). The digital DNA-DNA hybridization and average nucleotide identity values calculated from whole genome sequences between strain KNDSW-TSA6 and the two most closely related strains A. soli BL21 and A. delafieldii ATCC 17505 were below the threshold values of 70 and 95 % respectively. Thus, the data from the polyphasic taxonomic analysis clearly indicates that strain KNDSW-TSA6 represents a novel species, for which the name Acidovorax kalamii sp. nov. is proposed. The type strain is Acidovorax kalamii (=MTCC 12652=KCTC 52819=VTCC-B-910010).
 
Publisher Microbiology Society
 
Date 2018-05-01
 
Type Article
PeerReviewed
 
Relation http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002736#tab2
http://crdd.osdd.net/open/2107/
 
Identifier Pal, Deepika and Kaur, Navjot and Sudan, Sarabjeet Kour and Bisht, Bhawana and Krishnamurthi, Srinivasan and Mayilraj, Shanmugam (2018) Acidovorax kalamii sp. nov., isolated from a water sample of the river Ganges. International journal of systematic and evolutionary microbiology, 68 (5). pp. 1719-1724. ISSN 1466-5034