Record Details

Comparative de novo transcriptomic profiling of the salinity stress responsiveness in contrasting pearl millet lines

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/10847/
http://dx.doi.org/10.1016/j.envexpbot.2018.07.008
10.1016/j.envexpbot.2018.07.008
 
Title Comparative de novo transcriptomic profiling of the salinity stress responsiveness in contrasting pearl millet lines
 
Creator Shinde, H
Tanaka, K
Dudhate, A
Tsugama, D
Mine, Y
Kamiya, T
Gupta, S K
Liu, S
Takano, T
 
Subject Pearl Millet
Genetics and Genomics
Plant Physiology
 
Description Pearl millet (Pennisetum glaucum (L) R. Br.) is a staple crop for more than 90 million poor farmers. It is known for its tolerance against drought, salinity, and high temperature. To understand the molecular mechanisms underlying its salinity tolerance, physiological analyses and a comparative transcriptome analysis between salinity tolerant (ICMB 01222) and salinity susceptible (ICMB 081) lines were conducted under control and salinity conditions. The physiological studies revealed that the tolerant line ICMB 01222 had a higher growth rate and accumulated higher amount of sugar in leaves under salinity stress. Sequencing using the Illumina HiSeq 2500 system generated a total of 977 million reads, and these reads were assembled de novo into contigs corresponding to gene products. A total of 11,627 differentially expressed genes (DEGs) were identified in both lines. These DEGs are involved in various metabolic pathways such as plant hormone signal transduction, mitogen-activated protein kinase signaling pathways, and so on. Genes involved in ubiquitin-mediated proteolysis and phenylpropanoid biosynthesis pathways were upregulated in the tolerant line. In contrast, unigenes involved in glycolysis/gluconeogenesis and genes for ribosomes were downregulated in the susceptible line. Genes encoding SBPs (SQUAMOSA promoter binding proteins), which are plant-specific transcription factors, were differentially expressed only in the tolerant line. Functional unigenes and pathways that are identified can provide useful clues for improving salinity stress tolerance in pearl millet.
 
Publisher Elsevier
 
Date 2018-11
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10847/1/Comparative%20de%20novo%20transcriptomic%20pro%EF%AC%81ling%20of%20the%20salinity%20stress.pdf
Shinde, H and Tanaka, K and Dudhate, A and Tsugama, D and Mine, Y and Kamiya, T and Gupta, S K and Liu, S and Takano, T (2018) Comparative de novo transcriptomic profiling of the salinity stress responsiveness in contrasting pearl millet lines. Environmental and Experimental Botany (TSI), 155. pp. 619-627. ISSN 00988472