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Evaluation of paraprobiotic applicability of Lactobacillus plantarum in improving the immune response and disease protection in giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879)

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Relation http://eprints.cmfri.org.in/12627/
https://www.sciencedirect.com/science/article/pii/S1050464814004781
 
Title Evaluation of paraprobiotic applicability of Lactobacillus plantarum
in improving the immune response and disease protection in giant
freshwater prawn, Macrobrachium rosenbergii (de Man, 1879)
 
Creator Dash, Gyanaranjan
Raman, Ram Prakash
Pani Prasad, K
Makesh, M
Pradeep, M A
Dash, Swatipriyanka Sen
 
Subject Bioactive compound
Fish Diseases
 
Description Paraprobiotics, also known as ghost probiotics, are non-viable microbial cells which, when administered
in adequate amounts, confer a benefit on the host. However, the advantage of non-viable microbes over
their viable counterparts is a much debated topic in aquaculture. Therefore, the present study was
conducted to evaluate paraprobiotic effect of heat-killed Lactobacillus plantarum on giant freshwater
prawn Macrobrachium rosenbergii. A 90-day feeding trial was conducted by feeding prawn juveniles
(mean weight ± SE: 0.54 ± 0.03 g) with three experimental diets prepared by supplementing basal diet
(Crude protein: 38%; Gross energy: 387 kcal 100 g�1) with different concentrations of heat-killed probiotics
bacteria viz. HKPB1 (107 cfu g�1 diet), HKPB2 (108 cfu g�1 diet), HKPB3 (109 cfu g�1 diet) and
control diet (unsupplemented diet). In the present study, growth parameters viz.WG % and SGR and feed
utilization parameters viz. FCE, FCR and PER, though improved marginally in all experimental groups,
were found to be insignificant (P > 0.05) compared to the control. The immune parameters viz. total
hemocyte count (THC), phenol oxidase (PO) activity, respiratory burst (RB) activity and clearance efficiency
were significantly improved (P < 0.05) with concurrent decrease (P < 0.05) in cumulative mortality
against Aeromonas hydrophila challenge in all the experimental groups except for HKPB1, where PO
and RB activity did not improve significantly (P > 0.05) compared to the control. Among the experimental
groups, though the improvement in immune parameters was higher (P < 0.05) in HKPB2 and HKPB3
compared to HKPB1 and the control, no significant difference (P > 0.05) was observed between HKPB2
and HKPB3. The results obtained from the present study indicate that the application of heat-killed
L. plantarum at a concentration of 108 cfu g�1 diet, though not effective in augmenting the growth and
feed utilization parameters, can significantly improve immune parameters and disease resistance of
M. rosenbergii in the laboratory condition.
 
Publisher Elsevier
 
Date 2015
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/12627/1/Gyanaranjan%20Dash_2015_Fish%20and%20Shellfish%20Immunology_Evaluation%20of%20paraprobiotic%20applicability%20of%20Lactobacillus%20plantarum.pdf
Dash, Gyanaranjan and Raman, Ram Prakash and Pani Prasad, K and Makesh, M and Pradeep, M A and Dash, Swatipriyanka Sen (2015) Evaluation of paraprobiotic applicability of Lactobacillus plantarum in improving the immune response and disease protection in giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879). Fish & Shellfish Immunology, 43. pp. 167-174.