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Uncovering the Role of Mitochondrial Iron-sulfur (Fe-S) Cluster Biogenesis in Human Health and Disease

Electronic Theses of Indian Institute of Science

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Title Uncovering the Role of Mitochondrial Iron-sulfur (Fe-S) Cluster Biogenesis in Human Health and Disease
 
Creator Saha, Prasenjit Prasad
 
Subject Mitochondrial Iron Sulfur
Cluster Biogenesis
Human Health and Disease
Iron Sulfur Proteins
Fe-S Cluster Biogenesis
Fe-S Clusters
ISCU Myopathy
NFS1-ISD11
Iron-sulfur Cluster Scaffold Protein (ISCU)
Combined Oxidative Phosphorylation Deficiency 19 (COXPD19)
Mitochondrial Matrix Protein ISD11
Infantile Mitochondrial Complex II/III Deficiency (IMC23D)
Biochemistry
 
Description Mitochondrial dysfunction has been implicated for a wide range of human diseases. One of the major biosynthetic processes in human mitochondria is the biogenesis of Iron-Sulfur (Fe-S) clusters which primarily involves in electron transfer reactions during oxidative phosphorylation (OXPHOS). Defects in Fe-S cluster biogenesis process leads to mitochondrial dysfunction and that eventually results in various human mitochondrial disorders.
One of the major mitochondrial disorders associated with Fe-S cluster biogenesis impairment is exercise intolerance disorder ISCU myopathy, which is a result of loss of function of Fe-S cluster scaffold protein ISCU. Our biochemical results using yeast model system and HeLa cells lines suggests that ISCU Myopathy results in defective Fe-S cluster biogenesis in mitochondrial compartment. As a result, electron transport chain (ETC) complexes demonstrate significant reduction in their redox properties, leading to loss of cellular respiration. Furthermore, in ISCU Myopathy, mitochondria display enhancement in iron levels and reactive oxygen species, thereby causing oxidative stress leading to impairment in the mitochondrial functions. On the other hand, in mammalian mitochondria, the initial step of Fe-S cluster assembly process is assisted by NFS1-ISD11 complex, which delivers sulfur to the scaffold protein ISCU during Fe-S cluster synthesis. In humans, loss of ISD11 function leads to development of respiratory distress disorder, Combined Oxidative Phosphorylation Deficiency 19 (COXPD19). Our study maps the important ISD11 amino acid residues critical for in vivo Fe-S cluster biogenesis. Importantly, mutation of these critical ISD11 residues to alanine leads to its compromised interaction with NFS1, which results in reduced stability and enhanced aggregation of NFS1 in the mitochondria. Moreover, our findings highlight that, COXPD19 associated R68L ISD11 mutant displays reduced affinity to form a stable sub-complex with NFS1, thereby fails to prevent NFS1 aggregation, resulting impairment of Fe-S cluster biogenesis. The prime affected machinery is the ETC complex which demonstrates compromised redox properties, causing diminished mitochondrial respiration in COXPD19 patients.
In summary, our findings provide compelling evidence that respiration defect due to impaired biogenesis of Fe-S clusters in ISCU myopathy patients, leads to manifestation of complex clinical symptoms. Additionally, our study highlights the role of ISD11 protein in Fe-S cluster biogenesis and maps the surface residues of ISD11 protein that are involved in interaction with sulfur donor protein NFS1. Moreover, we have demonstrated the molecular basis of disease progression of COXPD19 as a result of R68L ISD11 mutation.
 
Contributor D'Silva, Patrick
 
Date 2018-09-04T05:13:34Z
2018-09-04T05:13:34Z
2018-09-04
2015
 
Type Thesis
 
Identifier http://etd.iisc.ernet.in/2005/4002
http://etd.iisc.ernet.in/abstracts/4888/G27225-Abs.pdf
 
Language en_US
 
Relation G27225