Record Details

Cloning and Expression of a Diagnostic Antigen for Invasive Amoebiasis

Electronic Theses of Indian Institute of Science

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Field Value
 
Title Cloning and Expression of a Diagnostic Antigen for Invasive Amoebiasis
 
Creator Shenai, Bhaskar R
 
Subject Limnology
invasive amoebiasis
Cloning-Invasive
E-histolytica
 
Description A crude extract of axenically grown amoebae was used as antigen in order to develop an AB microELISA for the detection and quantitation of E. histolytica-specific IgG antibodies. This ELISA was used to screen individual sera of patients suffering from invasive amoebiasis (n=47)and control individuals(n=33). Significant titers of E-histolytica- specific IgG antibodies were present only in sera of patients suffering from invasive amoebiasis. The AB-microELISA had a sensitivity of 98% and a specificity of 97%.


Immunoblot analysis of the crude E. histolytica extract indicated the presence of several antigenic proteins. One of the common antigenic proteins recognized by the individual patients' sera had a molecular weight of 160.170 kDa Sera of five patienta with high titers of E. histotytica-specific IgG antibodies were used to prepare a serum pool. This pooled serum was used for immunoscreening of an E. histolyitca cDNA expression library prepared in the phage vector l ZAP-II. A strongly immunoreactive phage done was identified, from which he recombinant phagemid was released by in vivo excision for characterization of the cDNA insert.
 
Publisher Indian Institute of Science
 
Contributor Subba Rao, P V
 
Date 2005-04-29T05:30:07Z
2005-04-29T05:30:07Z
2005-04-29T05:30:07Z
1994-08
 
Type Electronic Thesis and Dissertation
 
Format 4611050 bytes
application/pdf
 
Identifier http://etd.iisc.ernet.in/handle/2005/112
null
 
Language en
 
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