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Isolation and characterization of Aquaporin 1 (AQP1), sodium/ potassium-transporting ATPase subunit alpha-1 (Na/K-ATPase α1), Heat Shock Protein 90 (HSP90), Heat Shock Cognate 71 (HSC71), Osmotic Stress Transcription Factor 1 (OSTF1) and Transcription Factor II B (TFIIB) genes from a euryhaline fish, Etroplus suratensis

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Relation http://eprints.cmfri.org.in/13028/
https ://doi.org/10.1007/s1103 3-018-4350-1
https://doi.org/10.1007/s11033-018-4350-1
 
Title Isolation and characterization of Aquaporin 1 (AQP1), sodium/
potassium-transporting ATPase subunit alpha-1 (Na/K-ATPase α1),
Heat Shock Protein 90 (HSP90), Heat Shock Cognate 71 (HSC71),
Osmotic Stress Transcription Factor 1 (OSTF1) and Transcription Factor
II B (TFIIB) genes from a euryhaline fish, Etroplus suratensis
 
Creator Sebastian, Wilson
Sukumaran, Sandhya
Zacharia, P U
Gopalakrishnan, A
 
Subject Fish Genetics
 
Description The present study reports the complete sequences of Aquaporin 1 (AQP1) gene and partial sequences of genes, Sodium/
potassium-transporting ATPase subunit alpha-1 (Na/K-ATPase α1 subunit), Osmotic Stress Transcription Factor 1 (OSTF1),
Transcription Factor II B (TFIIB), Heat Shock Cognate 71 (HSC71) and Heat Shock Protein 90 (HSP90) obtained from
mRNA and genomic DNA of Etroplus suratensis. They are candidate genes involved in stress responses of fishes. AQP1
gene was 2163 bp long. Its mRNA sequence has 55 bp 5′ UTR, 783 bp open reading frame (ORF), 119 bp 3′ UTR, three
intronic regions and 90% identity with AQP1 of Oreochromis niloticus. The partial Na/K-ATPase α1subunit gene obtained
5998 bp length with an ORF of 2213 bp and 12 intronic regions. The partial OSTF1, TF IIB, HSC71 and HSP90 mRNA
sequences obtained were 1473 bp, 587 bp, 1708 bp and 151 bp in length respectively. All the genes showed a high sequence
similarity with respective genes reported from fishes. Comparison of AQP1 and Na/K-ATPase α1 genomic DNA sequence
of E. suratensis collected from different water system showed two type of AQP1 with one synonymous mutation in exon-1
and higher sequence difference in intronic regions (including addition, deletion, transition and transversion mutations) with
few synonymous and non-synonymous mutations in the exons of Na/K-ATPase α1. The sequence information of these major
candidate genes involved in stress responses will help in further studies on population genetics, adaptive variations and genetic
improvement programs of this cichlid species having aquaculture, ornamental and evolutionary importance.
 
Publisher Kluwer
 
Date 2018
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13028/1/Sandhya%20Sukumaran_Gopalakrishnan%20A_2018_Etroplus%20genes.pdf
Sebastian, Wilson and Sukumaran, Sandhya and Zacharia, P U and Gopalakrishnan, A (2018) Isolation and characterization of Aquaporin 1 (AQP1), sodium/ potassium-transporting ATPase subunit alpha-1 (Na/K-ATPase α1), Heat Shock Protein 90 (HSP90), Heat Shock Cognate 71 (HSC71), Osmotic Stress Transcription Factor 1 (OSTF1) and Transcription Factor II B (TFIIB) genes from a euryhaline fish, Etroplus suratensis. Molecular Biology Reports. pp. 1-7.