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Crystal structure of an inactive variant of the quorum-sensing master regulator HapR from the protease-deficient non-O1, non-O139 Vibrio cholerae strain V2.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Crystal structure of an inactive variant of the quorum-sensing master regulator HapR from the protease-deficient non-O1, non-O139 Vibrio cholerae strain V2.
 
Creator Cruite, Justin
Succo, Patrick
Raychaudhuri, Saumya
Kull, F Jon
 
Subject QR Microbiology
 
Description HapR is a TetR-family transcriptional regulator that controls quorum sensing in Vibrio cholerae, the causative agent of cholera. HapR regulates the expression of hemagglutinin protease, virulence and biofilm genes. The crystal structure of wild-type HapR from V. cholerae strain O1 El Tor C6706 has previously been solved. In this study, the structure of a DNA-binding-deficient variant of HapR (HapR) derived from the protease-deficient V. cholerae serotype O37 strain V2 is reported. The structure reveals no structural differences compared with wild-type HapR. However, structural alignment of HapR with the TetR-family member QacR in complex with its operator DNA suggests that the aspartate residue located between the regulatory and DNA-binding domains may clash with and electrostatically repel the phosphate backbone of DNA to prevent binding.
 
Publisher International Union of Crystellography
 
Date 2018-06-01
 
Type Article
PeerReviewed
 
Relation http://scripts.iucr.org/cgi-bin/paper?S2053230X18006519
http://crdd.osdd.net/open/2123/
 
Identifier Cruite, Justin and Succo, Patrick and Raychaudhuri, Saumya and Kull, F Jon (2018) Crystal structure of an inactive variant of the quorum-sensing master regulator HapR from the protease-deficient non-O1, non-O139 Vibrio cholerae strain V2. Acta crystallographica. Section F, Structural biology communications, 74 (Pt 6). pp. 331-336. ISSN 2053-230X