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<p class="TitleMain" style="text-align: justify;">Anti-nephrolithiatic potential and the protective role of saponin-rich extract of <em>Dianthus basuticus</em> against acetaminophen-induced damage in HEK293 cells</p><p style="text-align: justify;"><strong> </strong><strong><br /></strong></p>

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Title Statement <p class="TitleMain" style="text-align: justify;">Anti-nephrolithiatic potential and the protective role of saponin-rich extract of <em>Dianthus basuticus</em> against acetaminophen-induced damage in HEK293 cells</p><p style="text-align: justify;"><strong> </strong><strong><br /></strong></p>
 
Added Entry - Uncontrolled Name Nafiu, Mikhail Olugbemiro; University of the Free State, Qwaqwa Campus
Ashafa, Anofi Omotayo Tom; University of the Free State, Qwaqwa Campus
Sabiu, Saheed ; University of the Free State, Qwaqwa Campus
 
Uncontrolled Index Term Calcium oxalate; Dianthus basuticus; HEK293 cell; Hoechst 33342; MTT; Nephrolithiasis
 
Summary, etc. <p style="text-align: justify;">Saponins have found ethnomedicinal applications against a number of diseases including oxidative stress, urinary and kidney ailments. This study investigated antioxidant, anti-nephrolithiatic and nephroprotective properties of saponin-rich extract of <em>Dianthus basuticus</em> <em>in vitro</em>. While nephroprotective activity was demonstrated against acetaminophen-mediated toxicity in HEK293 cells using the MTT method, its inhibition of calcium oxalate (CaOx) nucleation was evaluated in the anti-nephrolithiatic study. The antioxidant effect was demonstrated against OH and lipid peroxide radicals. The significant (<em>p</em> &lt;0.05) acetaminophen-induced reduction of viable cells in the nephrotoxic cells following 24 hours incubation was reverted and cell mortality dropped from 81.98 to 13.15 % at 125 µg/mL dose of the extract.  The extract significantly inhibited CaOx nucleation crystals in a dose-related manner with the highest dose being 51.11 % potent. The inhibitory effect of the extract had an overall IC<sub>50</sub> of 1.03 µg/mL (R<sup>2</sup>= 0.9698) which is corroborated by the degree of turbidity of the treated crystals solutions. The overall effects elicited by the extract may be attributed to its antioxidant activity which was concentration-related with the highest dose exhibiting the most potent effect (IC<sub>50</sub>: 0.45–0.63 µg/mL) when compared with vitamin C (IC<sub>50</sub>: 0.49–0.69 mg/mL). Hence, <em>D. basuticus</em> is potentially a new lead agent in the management of nephrolithiasis and renal disorders.</p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2018-09-25 12:39:37
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/15784
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 9, ##issue.no## 2 (2018): June, 2018
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJNPR/article/download/15784/47235
 
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