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Isolation and phylogenetic identification of heterotrophic thraustochytrids from mangrove habitats along the southwest coast of India and prospecting their PUFA accumulation

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Relation http://eprints.cmfri.org.in/13035/
http://link-springer-com-443.webvpn.jxutcm.edu.cn/content/pdf/10.1007%2Fs10811-018-1627-7.pdf
https://doi.org/10.1007/s10811-018-1627-7
 
Title Isolation and phylogenetic identification of heterotrophic
thraustochytrids from mangrove habitats along the southwest coast
of India and prospecting their PUFA accumulation
 
Creator Jaseera, K V
Kaladharan, P
Vijayan, K K
Sandhya, S V
Leo Antony, M
Pradeep, M A
 
Subject Bioactive compounds
Mangroves
 
Description Among several sustainable resources, marine heterotrophic microorganisms have gained much interest for various commercial
applications owing to their potential to produce high quantities of polyunsaturated fatty acids. Above 70% of the globally
produced fish oil is utilised for aqua feed formulations. Intense research is being carried out to find suitable alternatives to fish
oil. Oleaginous protists are being explored as a promising substitute to fish oil and also for various high-value-added products.
However, characterisation and utilisation of these unique protists remain underexplored in many countries including India and
this prompted us to screen and isolate these heterotrophic microorganisms capable of producing PUFAs. In this study, 11 strains
of heterotrophic thraustochytrid capable of producing significant quantities of polyunsaturated fatty acids were successfully
isolated from different mangrove habitats along the southwest coast of India. Morphological characterisation along with molecular
identification revealed that these strains showed close similarity to Aurantiochytrium spp. of the family Thraustochytridae.
They are able to produce 7–11 g of dry biomass per litre of culture. Their fatty acid content was up to 12%(wt/wt) of biomass and
GC analysis confirmed that these protists can accumulate docosahexaenoic acid as high as 22–60% of the total fatty acids. Their
enzymatic activity, tolerance to various abiotic stress factors and antagonistic potential against vibrios are also described. These
heterotrophic protists can antagonise aquaculture pathogens and act as an excellent source of docosahexaenoic acid that indicates
their potential as a sustainable alternative to fish oil.
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13035/1/Kaladharan%20P_Jaseera%20K%20V_Vijayan%20K%20K_2018_Journal%20of%20Applied%20Phycology_Isolation%20and%20Phylogenetic%20Identification_.pdf
Jaseera, K V and Kaladharan, P and Vijayan, K K and Sandhya, S V and Leo Antony, M and Pradeep, M A Isolation and phylogenetic identification of heterotrophic thraustochytrids from mangrove habitats along the southwest coast of India and prospecting their PUFA accumulation. Journal of Applied Phycology. pp. 1-12.