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Yeast MRX deletions have short chronological life span and more triacylglycerols.

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Relation http://ir.cftri.com/12719/
http://dx.doi.org/10.1093/femsyr/fov109
 
Title Yeast MRX deletions have short chronological life span
and more triacylglycerols.
 
Creator Kanaga Vijayan, D.
Ram, Rajasekharan
Malathi, Srinivasan
 
Subject 22 Lipid Chemistry
19 Yeast
 
Description Saccharomyces cerevisiae is an excellent model organism for lipid research. Here, we have used yeast haploid RAdiation
Damage (RAD) deletion strains to study life span and lipid storage patterns. RAD genes are mainly involved in DNA repair
mechanism and hence, their deletions have resulted in shorter life span. Viable RAD mutants were screened for non-polar
lipid content, and some of the mutants showed significantly high amounts of triacylglycerol (TAG) and steryl ester, besides
short chronological life span. Among these, RAD50, MRE11 and XRS2 form a complex, MRX that is involved in homologous
recombination that showed an increase in the amount of TAG. Microarray data of single MRX deletions revealed that
besides DNA damage signature genes, lipid metabolism genes are also differentially expressed. Lipid biosynthetic genes
(LPP1, SLC1) were upregulated and lipid hydrolytic gene (TGL3) was downregulated. We observed that rad50�, mre11�, xrs2�
and mrx� strains have high number of lipid droplets (LDs) with fragmented mitochondria. These mutants have a short
chronological life span compared to wild type. Aged wild-type cells also accumulated TAG with LDs of ∼2.0 μm in diameter.
These results suggest that TAG accumulation and big size LDs could be possible markers for premature or normal aging.
 
Date 2016
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/12719/1/FEMS%20Yeast%20Research%2C%2016%2C%202016.pdf
Kanaga Vijayan, D. and Ram, Rajasekharan and Malathi, Srinivasan (2016) Yeast MRX deletions have short chronological life span and more triacylglycerols. FEMS Yeast Research, 16 (1). pp. 1-14.