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Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification

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Relation http://eprints.cmfri.org.in/13038/
http://epubs.icar.org.in/ejournal/index.php/IJF/article/view/81674
DOI: 10.21077/ijf.2018.65.3.81674-06
 
Title Development and validation of microsatellite markers in a protandrous
fish species Eleutheronema tetradactylum (Shaw, 1804) through
cross-species amplification
 
Creator Ismail, Shihab
Vineesh, N
Mohitha, C
Vijayagopal, P
Gopalakrishnan, A
 
Subject Fish Genetics
 
Description The four fingered threadfin Eleutheronema tetradactylum (Shaw, 1804) is a prioritised species for mariculture in India. Their demand in the domestic markets is rapidly growing. Genetic stock structure analysis of fish populations is an important aspect from fisheries management perspective. The present study was conducted to develop microsatellite primers through cross-priming to elucidate the genetic structure of E. tetradactylum. A total of 13 polymorphic microsatellite markers were developed from the resource species, Pacific salmon Polydactylus sexfilis. The observed mean and the effective number of alleles were found to be 11.962 and 6.927 respectively. The mean of observed heterozygosity (Ho) and expected heterozygosity (He) values obtained were 0.784 and 0.798 respectively. These new microsatellite markers can be used as effective tools for studying genetic disparity as well as for elucidating evolutionary relationships among E. tetradactylum populations.
 
Date 2018
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13038/1/Shihab_Gopalakrishnan_2018_IJF_65-3_Development%20and%20validation%20of%20microsatellite%20markers%20in%20a%20protandrous%20fish.pdf
Ismail, Shihab and Vineesh, N and Mohitha, C and Vijayagopal, P and Gopalakrishnan, A (2018) Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification. Indian Journal of Fisheries, 65 (3). pp. 47-51.