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Quality of lemongrass (cymbopogon flexuosus) oils under different storage conditions and their nematicidal activity

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Title Quality of lemongrass (cymbopogon flexuosus) oils under different storage conditions and their nematicidal activity
 
Creator Yogesh Kumar Singh
 
Contributor Madan, V. K.
 
Subject Cymbopogon flexuosus, Lemongrass, Essential oil quality, Storage effect, Nematicidal activity, Meloidogyne javanica, Meloidogyne incognita, Rotylenchulus reniformis
 
Description Laboratory studies were conducted for qualitative and quantitative estimation of essential
oils of lemongrass (Cymbopogon flexuosus) genotypes/varieties, effect of various storage methods
on quality of essential oil of variety CKP–25 and nematicidal activity of aqueous extracts of
essential oils of six genotypes/varieties of lemongrass against nematodes Meloidogyne javanica,
Meloidogyne incognita and Rotylenchulus reniformis. The essential oils were analysed by GLC
techniques employing Flame Ionization Detector (FID) equipped with Capillary Column Thermo
TR–Wax (Polyethylene glycol). For storage studies the essential oil was kept for six months in
glass, metal and plastic vials, replicated thrice under closed as well as open conditions. The quality
of oils kept in different vials was estimated just before storage and subsequently after 2, 4 and 6
months of storage. The retention times for citral–a (geranial), citral–b (neral), geraniol and
citronellal were 16.42, 15.53, 17.84 and 12.35 minutes, respectively. The oil content on Fresh
Weight Basis (FWB) was in the range from 0.24 to 0.74%. The range of percent composition of
various major and minor constituents were as follows: citral–a (47.1–52.4%), citral–b (30.3–37.5%),
total citral (82.7–84.8%), geraniol (2.5–2.8%) and citronellal (0.8–1.1%). Citral–a (geranial), citral–
b (neral), total citral, geraniol and citronellal contents in the essential oils decreased during storage
period. The decrease in citral–a (geranial), citral–b (neral), total citral, geraniol and citronellal
contents during storage was minimum in glass vials followed by plastic vials and metal (aluminium)
vials. Between closed and open vials, the decrease in citral–a, citral–b, total citral, geraniol and
citronellal contents was less in closed vials in comparison to open vials. The probable reason for
degradation may be due to oxidation of citral–a, citral–b, geraniol and citronellal into their
derivatives resulting in less concentration of these constituents in the essential oil. The nematicidal
activity of aqueous extracts of essential oils of lemongrass was highest against the nematodes M.
incognita followed by M. javanica and Rotylenchulus reniformis. The probable reason for
nematicidal activity of aqueous extracts of lemongrass essential oils may be due to the presence of
aldehydic group in major constituents (citral–a, citral–b) & minor constituents (citronellal) and
alcoholic group (geraniol) in minor constituents. Keeping in view the storage studies under
laboratory conditions, it can be inferred that essential oils of lemongrass can be kept in closed glass
vials for less degradation in quality of essential oil and also these essential oils are effective in
controlling the nematodes under laboratory conditions.
 
Date 2016-12-01T13:46:02Z
2016-12-01T13:46:02Z
2006
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/88628
 
Language en
 
Format application/pdf
 
Publisher CCSHAU