Physiological and growth responses of arbuscular mycorrhizal (Glomus mosseae) inoculated Dalbergia sissoo Roxb. seedlings to water stress
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Title |
Physiological and growth responses of arbuscular mycorrhizal (Glomus mosseae) inoculated Dalbergia sissoo Roxb. seedlings to water stress
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Creator |
Garhwal, Amit
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Contributor |
Beniwal, R.S.
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Subject |
Planting, Irrigation, Application methods, Weather hazards, Fungi, Vegetative propagation, Biological development, Chlorophylls, Dalbergia sissoo, Biological phenomena
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Description |
In August 2013, an experiment on physiological and growth responses of arbuscular mycorrhizal (Glomus mosseae) inoculated Dalbergia sissoo Roxb. seedlings to water stress was conducted at the Department of Forestry, CCSHAU, HISAR (Haryana) to investigate the effect of water stress on various physiological and growth parameters and to observe the effect of AM fungi in overcoming the water stress. Three months old AM-inoculated and non-inoculated shisham seedlings were subjected to water stress for 25 days by withholding the water till the drooping of leaves that coincides with the significant decline in chlorophyll fluorescence yield. Water stress in shisham seedlings had significant adverse effect on the AM abundance in roots, however, re-watering to them for 7 days resulted in the improvement of AM colonization. Water stress also caused significant decrease in plant fresh and dry weight, collar diameter, shoot length, root length, no. of leaves and leaf area in both AM and non-AM plants, however, the decreases were significantly less in AM plants than non-AM plants. AM-inoculation helped in maintaining significantly more periodic increment in root collar diameter and shoot length in AM plants than non-AM plants during the period of water stress (25 days) and re-watering (7 days). Water stress promoted leaf shedding in all the stressed shisham seedlings, but, it was comparatively high in un-inoculated control stressed seedlings. Water stress also caused reductions in chlorophyll and carotenoid pigments, chlorophyll fluorescence yield, relative water content, however, reductions in these parameters were significantly more in un-inoculated water stressed seedlings compared to their AM-inoculated counterparts. Stress signal compounds like proline and total sugar concentrations in leaves increased significantly in response to water stress, whereas, the protective effects of arbuscular mycorrhizae helped the water stressed shisham seedlings through keeping at low level the proline and sugar accumulation in AM plant leaves than non- AM leaves is a step to avoid stress. During the period of 7 days re-watering, recovery in fluorescence yield was faster in AM-inoculated seedlings compared to un-inoculated counterparts which suggest that the decline in PS II efficiency is regulatory, serving a photo-protective role and can be a useful screening test of water stress in shisham. Although water stress caused decreases in mycorrhizal abundance, AM-inoculation substantially protected plant growth performance compared with non-AM shisham. These results suggest that the Glomus mosseae inoculation greatly assists the plants under water stress and helps in making these effects moderate. These results are more important from the points of view of afforestation in drought prone conditions. |
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Date |
2016-10-05T10:01:42Z
2016-10-05T10:01:42Z 2014 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/79955
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Language |
en
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Format |
application/pdf
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Publisher |
CCSHAU
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