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Isolation and characterization of sulphur oxidizing bacteria

KrishiKosh

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Title Isolation and characterization of sulphur oxidizing bacteria
 
Creator Aastha
 
Contributor Goyal, Sneh
 
Subject Sulphur oxidizing bacteria, Sulphide Oxidase, Sulphate production, Acterial isolates
 
Description Sulphur is considered as the fourth major plant nutrient after N, P and K and is one
of the sixteen nutrient elements, which are essential for the growth and development of
plants especially in the agricultural crop production. Sulphur undergoes a number of
biological transformations in nature carried out exclusively by microorganisms. The
transformations of inorganic sulphur compounds in nature have been formalized in the
sulphur cycle. Sulphur oxidation is the most important step of sulphur cycle which improves
soil fertility. It results in the formation of sulphate, which can be used by the plants while
the acidity produced by oxidation helps to solubilize plant nutrients and improves soil.
Microorganisms that are capable of oxidizing reduced inorganic sulphur compounds with
sulphate as the end product are known as sulphur oxidizing bacteria (SOB) .
A total of 65 bacterial isolates were isolated from rhizospheric soils of mustard
crops and decomposing sites. Out of 65 bacterial isolates, 55 isolates were screened on the
basis of bromo cresol purple dye reduction test. Among 55 isolates, 32 bacterial isolates
were further screened for their ability to produce sulphate by barium chloride test. The two
isolates SOB98 and SOBD42 showed maximum sulphate production 2.631 and 2.331mM
respectively. These isolates were selected for optimization of the cultural conditions for the
sulphate production. Glucose was found to be the best carbon source and ammonium
chloride was the best nitrogen source. It was observed that 30ºC was the optimum
temperature for sulphate production by isolate(s) SOB98 and SOBD42. Sulphate production
by isolate SOB98 and SOBD42 was maximum after 7 days of incubation and at pH 6 and
pH4, respectively. Sulphide oxidase activity of isolates SOB98 and SOBD42 were 32.3 and
26.9 U/ml, respectively. On the basis of morphological and biochemical tests the promising
isolates probably belonged to genera Xanthobacter sp. (SOB98) and Pseudomonass sp.
(SOBD42).
 
Date 2016-09-26T10:05:49Z
2016-09-26T10:05:49Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/78790
 
Language en
 
Format application/pdf
 
Publisher CCSHAU