Record Details

MOLECULAR AND PHENOTYPIC ANALYSIS OF Brassica juncea TRANSGENICS HAVING DEFENSE REGULATORY NPR1 GENE

KrishiKosh

View Archive Info
 
 
Field Value
 
Title MOLECULAR AND PHENOTYPIC ANALYSIS OF Brassica juncea TRANSGENICS HAVING DEFENSE REGULATORY NPR1 GENE
M Sc
 
Creator RAJENDRA PRASAD MEENA
 
Contributor Anita Grover
 
Subject marketing, grain, economics, wheats, distribution systems, supply, sugar, wells, hydrocarbons, grain legumes
 
Description T-8304
Alternaria blight is one of the serious diseases of Brassica juncea causing 45-58% loss in the
yield. Manipulating defense regulatory genes [eg. No PR1 (NPR1) gene] is one of the strategies
being followed internationally. In order to develop resistance against Alternaria brassicae,
nineteen putative transgenic lines of Brassica juncea having NPR1 gene under control of 35S
promoter were developed earlier. In the present study, these transgenic lines were confirmed for
gene integration and expression and its effect on resistance against Alternaria. Gene integration
was confirmed by doing PCR with NPT II primers. In order to reconfirm the NPR1 gene
integration, PCR was done using 35S forward primer and NPR1 reverse primer. All the nineteen
putative lines were found to be positive. RT-PCR using NPR1 primers were done to check levels
of gene expression. Eleven lines (1,5,6,7,8,9,11,12,14,16,18) were found to be over expressing
NPR1 about 5 fold over nontransgenic control while other eight lines (2,3,4,10,13,15,17,19) did
not show such high expression (only 2 fold). Seven lines encompassing both high NPR1
expressing and not so high NPR1 expressing, were analyzed for disease resistance. Resistance
was scored in terms of time of onset of symptoms and size and number of lesions. The symptoms
of infection appeared after day 3 and 4 of post inoculation in control plants while it was after day
6 and 7 of post inoculation in transgenic plants. The levels of resistance in-vitro varied from 2 to
2.5 fold as compared to the control. In-vivo assays showed 2 to 3 fold resistance in transgenic
plants as compared to control plants. It was found that expression level of NPR1 is directly
related to levels of resistance
 
Date 2016-10-19T10:57:03Z
2016-10-19T10:57:03Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/80975
 
Format application/pdf
 
Publisher IARI, NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY