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MORPHOLOGICAL AND MOLECULAR CHARACTERISATION OF TRICHODERMA SPECIES

KrishiKosh

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Title MORPHOLOGICAL AND MOLECULAR CHARACTERISATION OF TRICHODERMA SPECIES
 
Creator RAJESH ANASOSALU MARIGOWDA
 
Contributor T. Prameela Devi
 
Subject fungi, diseases, genetic structures, developmental stages, dna, land resources, pcr, fruits, productivity, nucleotides
 
Description T-8119
The identification of Trichoderma species, existing in different fields of I.A.R.I., New
Delhi was done by using morphology and molecular based techniques. Twenty soil
samples were collected and isolations were made. Twelve isolates of Trichoderma were
obtained from Trichoderma selective medium selected on the basis of genus characters.
These twelve isolates were made into two different sections and five different groups on
the basis of their colony characters viz., colony growth, color and texture and
microscopic observations viz., conidiophore branching, phialides (shape and
disposition), conidia (color, shape, size and wall) and chlamydospores. The characters of
these isolates were compared with the reported literature and confirmed the group I
isolates as Trichoderma pseudokoningi, group II as Trichoderma reesei.., group III as
Trichoderma longibrachiatum, group IV as Trichoderma virens and group V as
Trichoderma hamatum. PCR amplification and sequence analysis of r-DNA region (ITS
1-5.8s–ITS 2) and a small fragment of translation elongation factor (tef-1) gene were
used to characterize these twelve isolates of Trichoderma. The respective universal
primers (for ITS and tef-1 regions amplification) were successfully used to amplify
genomic DNA extracted from mycelium of these isolates and sequence analysis showed
clear difference among the isolates. Morphological grouping and speciation matched
with molecular grouping of most of the isolates especially with the results of ITS region
based analysis. The integration of morphology and molecular based techniques gave fair
solution in easing some of the complex problems that are associated with either
morphology or molecular based techniques.
 
Date 2016-12-17T13:51:38Z
2016-12-17T13:51:38Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/90770
 
Format application/pdf
 
Publisher IARI, DIVISION OF PLANT PATHOLOGY