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EVALUATION OF ANTIOXIDANT AND OTHER BENEFICIAL ACTIONS OF OMEGA 3 FATTY ACIDS AGAINST INDUCED PHYSICAL STRESS IN RATS

KrishiKosh

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Title EVALUATION OF ANTIOXIDANT AND OTHER BENEFICIAL ACTIONS OF OMEGA 3 FATTY ACIDS AGAINST INDUCED PHYSICAL STRESS IN RATS
 
Creator Y. JAYASUDHA
 
Contributor Dr. K. Aswani kumar
 
Subject EVALUATION OF ANTIOXIDANT AND OTHER BENEFICIAL ACTIONS OF OMEGA 3 FATTY ACIDS AGAINST INDUCED PHYSICAL STRESS IN RATS
 
Description Stress is perceived by the brain and results in adaptive responses in other organ
systems via neural and neuroendocrine pathways. Stress responses are readily
identifiable for many routinely evaluated parameters (e.g., leukocyte counts) and organ
systems that are sensitive to stress. The present study was aimed to evaluate the
antioxidant and other beneficial effects of omega-3 fatty acids in physical stressinduced
rats, which were divided into four groups as: Group I: Normal control, II: Rats
with physical stress , III: Normal Rats fed with omega 3 fatty acid (fish oil) diet and
Group IV: Rats with physical stress and omega 3 fatty acid diet.
Average body weights were recorded at weekly intervals and blood collected for
haematology at weekly intervals. On 29th day, liver tissue was collected for estimation
of TBARS, protein carbonyls, GPx, SOD and GSH in liver tissue homogenates. Blood
was collected for serum separation at fortnight intervals and estimated total cholesterol,
TGs, HDL cholesterol, (LDL cholesterol calculated), serum troponins, creatinine, ALT,
glucose and total protein. Histopathology of heart, liver and kidney was also studied at
the end of the experiment.
Stress group (Group II) rats showed significant (P < 0.05) decrease in body
weight, lymphocytes, monocytes, basophils, total protein, HDL cholesterol, GSH, SOD
and GPx (324.45±0.84, 7.15±0.06, 0.30±0.00, 0.01±0.00, 6.72±0.14, 33.38±1.13,
10.80±0.00, 11.04±0.12 and 0.13±0.01 respectively), while TBARS, protein carbonyls,
TEC, Hb, PCV, eosinophils, neutrophils, total cholesterol, Triglyceride, LDL
cholesterol, serum creatinine, ALT, and blood glucose were significantly (P < 0.05)
increased in Stress Group rats (Group II) (0.69±0.00, 3.09±0.10, 9.60±0.18, 15.9±0.17,
48.33±0.53, 0.96±0.14, 3.46±0.03, 92.02±0.86, 80.18±1.09, 42.61±1.75, 0.83±0.01,
48.48±0.88 and 85.47±0.54 respectively).From these values it is evident that there is
considerable amount of stress induced in the rats of Group II. Group I did not reveal
any abnormalities on histopathology. Group II showed marked congestion and
degenerative changes in kidney and liver. The results mentioned above suggest that
physical stress causes oxidative stress via ROS production. Physical stress + fish oil
treated group (Group IV) showed significant (P < 0.05) improvement in body weight,
lymphocytes count, monocytes count, total protein, HDL cholesterol, GSH, SOD and
GPx. Group IV showed significant (P < 0.05) decrease in TBARS, protein carbonyls,
Hb, PCV, eosinophils count, neutrophils count, total cholesterol, Triglyceride, LDL
cholesterol, serum creatinine, ALT, and blood glucose. Group IV did not differ
significantly in some parameters like TEC, serum troponins and TLC compared to
group II.
From this study, it is evident that physical stress resulted in Oxidative damage to
kidney and liver and these effects can be ameliorated by supplementation of fish oil in
the diet.
 
Date 2017-01-04T16:11:37Z
2017-01-04T16:11:37Z
2016-02-02
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94550
 
Relation D;454
 
Format application/pdf
 
Publisher PVNR TVU