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Analyses of stability, diversity and inheritance of quality traits in Indian wheat (Triticum aestivum L.) germplasm

KrishiKosh

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Title Analyses of stability, diversity and inheritance of quality traits in Indian wheat (Triticum aestivum L.) germplasm
Ph.D.
 
Creator SUTAPA SARKAR
 
Contributor Anju M. Singh
 
Subject SSR, microsatellites, molecular markers, genetic diversity, wheat
 
Description T-8756
Genetic diversity of 35 wheat cultivars was evaluated using 30 polymorphic
microsatellite (SSR) markers. Analysis of molecular diversity revealed moderate
levels of polymorphisms in the set of genotypes studied. The 30 microsatellite
markers detected ninety alleles with an average of 03 alleles per locus. The allelic
polymorphic information content (PIC) value ranged from 0.06 to 0.76 with an
average of 0.45. The primers like Xgwm294, Xgwm146, Xgwm455, Xgwm497,
Xgwm136 and Xgwm132 could be considered particularly informative, as they
revealed four or more alleles per locus and displayed high PIC values. Ten rare and
three unique alleles were recorded among the 35 genotypes. The genetic
dissimilarity indices showed that the two most closely related cultivars were
‘Raj3765’ and ‘HD 2402’ displaying the lowest dissimilarity index, whereas highest
genetic distance was observed between ‘Pissi Local’ and HB 208. The land race
‘Pissi Local’ revealed very high genetic distance with most of the cultivars where the
dissimilarity indices ranged from 0.42-0.74. The dendrogram derived by using the
clustering of individuals using a genetic-distance based program, revealed three
major clusters comprising of 13, 12 and 10 genotypes respectively. Despite of the
presence of variability among the different cultivars of the country, the varieties
developed at various institutes in different time periods formed clusters indicating
the use of related genotypes in various institutes for wheat breeding programme. The
microsatellite markers were also able to differentiate different genotypes in a similar
pattern as on the basis of pedigree except in a few cases.
 
Date 2016-08-26T15:01:14Z
2016-08-26T15:01:14Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73941
 
Format application/pdf
 
Publisher IARI,Division of Genetics