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Studies on detection of shiga toxin-producing Escherichia coli in meat and meat products by multiplex polymerase chain reaction and their public health significance

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Title Studies on detection of shiga toxin-producing Escherichia coli in meat and meat products by multiplex polymerase chain reaction and their public health significance
 
Creator Rao, T. Srinivasa
 
Contributor Gill, J.P.S.
 
Subject E. coli
STEC
Multiplex PCR
Antibiotic resistant patterns resistance genes
serotyping
 
Description The present study was undertaken for isolation and characterization of shiga
toxin-producing Escherichia coli (STEC) isolates from meats and meat products by
multiplex PCR (m-PCR). To assess the public health significance of STEC water
and human stool samples were also analyzed. Out of 675 samples subjected for
m-PCR, 203 samples (30.07%) were positive for STEC. About 40.56 per cent of
raw meat samples, 11.37 per cent of RTE meat products, 28.12 per cent of water
and 13.46 per cent of human diarrhoeal stool samples were STEC positive. All 203
(100%) STEC carried stx1, 65.02 per cent, 44.33 per cent 66.01 per cent STEC
isolates carried stx2, eaeA and hlyA gene, respectively. Forty two percent of STEC
isolates showed haemolysis on blood agar plates, 83.74 per cent isolates exhibited
congo red binding activity and 99.01 per cent isolates showed vero cell cytotoxicity.
Seventy two STEC isolates were resistant to more than 10 (50%) of the antibiotics
tested. The most common tetracycline resistance genes were tetA (107 of 178;
60%) and tetB (48 of 106; 27%). Twelve percent of STEC isolates were carried
both tetA and tetB resistance genes. Only one isolate from chicken sausage has shown tetC resistance gene. Forty seven percent of STEC isolates harboured both
strA and strB genes. Thirty three percent and 10 per cent isolates carried strA and
strB gene, respectively and 10 per cent of isolates did not carry any gene. Cloning
and sequencing of tetA and tetB genes of O69 serotype isolated from buffalo meat
was done. The sequences were submitted to GenBank and the sequences were
(98-100%) identical with the published sequences. Sixteen serogroups (O4, O7,
O8, O9, O15, O20, O22, O91, O103, O105, O110, O113, O126, O148, O153 and
O168) which were isolated from human cases were also isolated from different
foods.
 
Date 2016-07-21T10:16:32Z
2016-07-21T10:16:32Z
2009-07-20
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69090
 
Language en
 
Format application/pdf