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Impact of biochar on rhizospheric bacterial community structure

KrishiKosh

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Title Impact of biochar on rhizospheric bacterial community structure
 
Creator Sihag, Khushboo
 
Contributor Rakesh Kumar
 
Subject Carbon, dna, Productivity, Sampling, Inorganic compounds, pcr, Electrophoresis, Planting, soil sampling, Biomass
 
Description Biochar is a fine-grained, carbon rich, porous product remaining after plant biomass has been
subjected to thermo-chemical conversion process (pyrolysis) at low temperatures in an environment with
little or no oxygen. There is a wide variety of char products produced industrially. Biochar is added to soil
with the intention to improve soil functions and to reduce emissions from biomass that would otherwise
naturally degrade to greenhouse gases. It has been shown to change soil microbial community and
composition which leads to change in functional ecology of microbial diversity. The composition of
bacterial community in soils high in black C or biochar differs significantly from that of unmodified soils
with the same mineralogy. Biochar amended soil samples were collected from IARI, New Delhi and a
control soil without biochar was taken from the adjoining field. Physico-chemical properties of the soil
sample such as EC, pH, field capacity moisture content, total N, P and K were estimated using standard
procedures. It was observed that EC, pH, field capacity moisture content were increased after addition of
biochar from 0.15 to 0.21, 7.4 to 7.6 and 24 to 39%, respectively. Microbial community structure analysis
was performed using PCR-DGGE method. Soil DNA was extracted from the soil sample using Zymo
Research Soil DNA Kit. Extracted DNA was amplified using 16S rDNA universal primers (27F
&1378R). Seminested PCR was conducted using 986 F primer with GC clamp and 1378 R primer.
Amplified 16S rDNA product was used as template DNA. The GC clamped 16S rDNA was run on
DGGE (35-65% Denaturant). Most prominent bands in DGGE were eluted out following the prescribed
protocol of Gene JET Gel Extraction Kit, purified and sequenced to identify the community using
BLAST from NCBI. Dendrogram was constructed using NT-Sys software based on the banding pattern
of DGGE. There was 80% similarity among non-amended biochar soil and 35% similarity with biochar
amended soil suggesting wide diversity. Four prominent bands were eluted using Gene JET Gel
Extraction Kit and sequenced. Present study concludes that the prominent microbial community belongs
to uncultured Proteobacteria and Proteobacterium spp. In control soil, many uncultured bacterium spp.
were found and Bacillus thuringenesis was found in biochar amended soil.
 
Date 2016-10-22T15:16:33Z
2016-10-22T15:16:33Z
2016
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/81411
 
Language en
 
Format application/pdf
 
Publisher CCSHAU