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Evaluation of retinopathic potential of fluoroquinolones

KrishiKosh

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Title Evaluation of retinopathic potential of fluoroquinolones
 
Creator Khan, Adil Mehraj
 
Contributor Rampal, S.
 
Subject Rabbits
Levofloxacin
Pazufloxacin
Enrofloxacin
Meloxicam
Retina
 
Description The present investigation was undertaken to evaluate the fluoroquinolones; levofloxacin, pazufloxacin and enrofloxacin for their retinopathic potential. Thirty two rabbits were divided to eight groups of four rabbits each. One group as control was administered requisite amount of vehicle 5 % dextrose and benzyl alcohol. Other groups were gavaged individual fluoroquinolones alone and in combination with meloxicam. Levofloxacin and pazufloxacin was administered at dose rate of 10 mg/Kg body weight b.i.d., whereas enrofloxacin and meloxicam were administered at 20 mg/Kg s.i.d. and 0.2mg/Kg s.i.d., respectively. Pharmacokinetics of these fluoroquinolones was studied after first dose and elimination kinetics parameters were calculated. Blood samples were collected at 3, 6, 12 and 24 hours after dosing. Procedure was repeated after 21 days of treatment. Blood samples were also collected at 7, 14 and 21st day of treatment for the estimation of plasma fluoroquinolone concentrations. The toxicokinetic evaluation of fluoroquinolones revealed that repeated administration produced a significant alteration in elimination phase parameters. The co-administration of meloxicam did not significantly change kinetic behaviour of these drugs. Evaluation of various enzymatic and non-enzymatic indices of antioxidant status revealed significant oxidative stress in pazufloxacin and enrofloxacin treated groups. The histological examination of retina revealed severe retinal damage in enrofloxacin and least in levofloxacin treated rabbits. The retinal lesions included retinal pigment epithelium thinning, retinal detachment, neovascularization and formation of apoptotic bodies in different retinal layers. Immunohistochemistry of ABCG-2 transporter protein revealed highest immunolocalization in enrofloxacin and lowest in the levofloxacin treated rabbits. Co-administration of meloxicam resulted in induction of the transporter protein. From the study it can be concluded that severity of retinopathic effect varies among fluoroquinolones.
 
Date 2016-08-26T16:25:34Z
2016-08-26T16:25:34Z
2014-02-19
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73977
 
Language en
 
Format application/pdf