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Biochemical studies in wheat (Triticum aestivum L.) seedlings under heat stress

KrishiKosh

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Title Biochemical studies in wheat (Triticum aestivum L.) seedlings under heat stress
 
Creator Reena
 
Contributor Madan, Shashi
 
Subject Tolerance, Enzymes, Genotypes, Biological phenomena, Wheats, Inorganic compounds, Planting, Chlorophylls, Antioxidants, Proteins
 
Description The present investigation was carried out to investigate the effect of heat stress and
revival on some antioxidative enzymes, metabolites, chlorophyll stability and polypeptide
pattern in the leaves of Wheat seedlings of heat susceptible (WH-147 and HS-277) and heat
tolerant (WH-1021 and HW-2045) genotypes. Seven days old seedlings grown at 25ºC were
exposed to 40ºC for 6 h and these seedlings were brought to 25ºC. The observations were
recorded in the leaves of control, stressed and revived seedlings on 2nd and 4th day of revival.
Screening of thirty six genotypes for thermo-tolerance was done based on wilting of primary
leaf and values of chlorophyll fluorescence. Chlorophyll stability (%) declined in tolerant and
susceptible genotypes under heat stress. There was enhancement in the activities of
antioxidative enzymes, viz. CAT, POX, GR and APX in the leaves of tolerant and susceptible
genotypes under heat stress, however, higher per cent increase was observed in tolerant
genotypes. Heat stress increased the SOD activity in tolerant genotypes but activity declined in
susceptible genotypes. On revival, the activities of the CAT, POX and GR declined in
comparison to stressed seedlings but remained higher as compared to control. Ascorbate
peroxidase activity remained higher on 2nd day and 4th day of revival in all the genotypes. The
higher accumulation of H2O2, ascorbic acid and malondialdehyde content was observed under
heat stress in all the genotypes but their level was more in heat susceptible genotypes as
compared to heat tolerant genotypes. SDS-PAGE of seedlings under stress conditions revealed
the appearance of polypeptide bands of different molecular weight in tolerant and susceptible
genotypes, and these polypeptides bands disappeared on revival of 2nd and 4th day. Based on
genotypes screening and antioxidant enzyme activities, out of four genotypes HW-2045 was
found to be the most tolerant and WH-147 as the most susceptible genotype.
 
Date 2016-11-16T10:04:47Z
2016-11-16T10:04:47Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85774
 
Language en
 
Format application/pdf
 
Publisher CCSHAU