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Studies on gene expression for Heat Shock Protein 70 and identification of sperm membrane proteins in relation to semen quality and fertility in buffalo bulls

KrishiKosh

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Title Studies on gene expression for Heat Shock Protein 70 and identification of sperm membrane proteins in relation to semen quality and fertility in buffalo bulls
 
Creator Kumar, Ajeet
 
Contributor Singh, Jagir
 
Subject Buffalo bulls
Conception rate
Fertility Associated Antigens (FAA)
Heat Shock Protein 70 (HSP70)
Sperm membrane proteins
 
Description The present study was conducted to investigate Heat Shock Protein 70 and
sperm membrane proteins in relation to semen quality and fertility in buffalo bulls. Frozen
semen from 20 buffalo bulls was collected, sperm motion traits were analyzed through
Computer Assisted Semen Analysis (CASA), live sperm count and HOS reactive sperms
were assessed. The total RNA was harvested from Percoll washed sperm with Trizol
reagent. The first strand cDNA was synthesized and HSP70 was quantified using a Real
time PCR. To predict fertility, bulls were ranked on the basis of semen evaluation traits.
Fertility Associated Antigens (FAA) were investigated in fresh semen using Reprotest
(Lateral Flow Cassettes). Sperm membrane proteins were harvested from frozen semen
using protein extraction buffer and SDS-PAGE was carried out by loading 100μg proteins
on 10% SDS polyacrylamide gels. The gel was stained with Coomassiae stain and the
protein bands were imaged using Syngene Gel Doc (Synoptic Ltd) and band analysis was
carried out using Gene Snap Image Acquisition Software. HSP70 was also investigated in
beef bull sperm membrane proteins through western blotting technique.
Buffalo bulls were divided into 3 groups on the basis of conception rates as
good fertility (63.30%), average fertility (37.72) and poor fertility (17.22%). The average
conception rate in good fertility bulls was significantly (p
 
Date 2016-07-21T09:53:32Z
2016-07-21T09:53:32Z
2011-01-11
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69083
 
Language en
 
Format application/pdf