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TO STUDY THE PREVALENCE OF acdS GENE IN RHIZOBIA OF TROPICAL LEGUMES

KrishiKosh

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Title TO STUDY THE PREVALENCE OF acdS GENE IN RHIZOBIA OF TROPICAL LEGUMES
M Sc
 
Creator VIKAS SHARMA
 
Contributor K. Annapurna
 
Subject manpower, sampling, selection, costs, processed products, topping, biological phenomena, adaptation, productivity, technological changes
 
Description T-8329
A collection of 115 root nodule bacteria from three different locations and six
different legumes viz: Pea, Lentil, Frenchbean, Soybean, Fenugreek and Chickpea were
screened for nodulation and assayed for 1-aminocyclopropane-1- carboxylate (ACC)
deaminase activity. 113 isolates of the total 115 root nodule bacteria tested positive for
nodule formation on their respective homologous legume hosts. There was a large
variation in the nodule number and nitrogenase activities among the isolates, with pea
and lentil rhizobia being most efficient. Polymerase chain reaction (PCR) was used to
rapidly isolate ACC structural genes from all 115 rhizobial isolates; only 41 were found
positive for the PCR reaction amplifying the acdS gene of the appropriate size of around
1000 bp. PCR-RFLP of selected acdS genes with three restriction enzymes AluI, Msp and
Mbo from the 6 legumes was found to have the same restriction pattern, indicating the
conservedness of the gene sequence in these rhizobia. However, enzyme activity or gene
expression varied among the isolates under in vitro conditions.
 
Date 2016-10-22T11:07:30Z
2016-10-22T11:07:30Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/81365
 
Format application/pdf
 
Publisher IARI, DIVISION OF MICROBIOLOGY