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GROWTH, PREDATORY ACTIVITY AND MOLECULAR SIGNATURE OF EGG PARASITIC FUNGI VERTICILLIUM CHLAMYDOSPORIUM AND PAECILOMYCES LILACINUS

KrishiKosh

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Title GROWTH, PREDATORY ACTIVITY AND MOLECULAR SIGNATURE OF EGG PARASITIC FUNGI VERTICILLIUM CHLAMYDOSPORIUM AND PAECILOMYCES LILACINUS
 
Creator DE, SUMANTA
 
Contributor Sanyal, P.K.
 
Subject fungi, biological development, polysaccharides, biological phenomena, eggs, concentrates, inorganic acid salts, poultry equipment, biological interaction, biological control
 
Description The present study indicated that maximum growth of Paecilomyces lilacinus was obtained in Corn Meal Agar compared to any other media types. Potato Dextrose Agar, Sabouraud Glucose Agar and Nutrient Agar provided similar growth rates. The preferred medium of growth for Verticillium chlamydoaporium was Corn Meal Agar followed by Potato Dextrose Agar. Nutrient Agar and Sabouraud Glucose Agar provided much lower but uniform growth. After an initial growth at 16 hour of incubation, no growth was observed in Water Agar for both the fungi. Colonies of P. lilacinus were dark brown to brownish black in colour while those of V. chlamydosporium were creamy to light brown. Colonies consisted of a basal felt with a floccose overgrowth of aerial mycelium. Six different temperatures, viz., 4o, 10o, 18o 26o, 34o and 40oC, were used to observe growth profiles of the fungi in Corn Meal Agar medium. While no and very little growth of P. lilacinus and V. chlamydosporium in terms of colony diameter were observed at 40 and 100C, respectively, growth profiles of both the fungi were optimum at 26-400C. Five different pH, viz., 4, 5, 6, 7 and 8, were set in Corn Meal Agar medium to observe growth profiles of the fungi. A varied range of pH (pH 4-8) supported growth of P. lilacinus and V. chlamydosporium in terms of colony diameter. The colony diameter of P. lilacinus and V. chlamydosporium was measured in presence various heavy metals. No statistically significant differences in colony diameters of P. lilacinus and V. chlamydosporium were observed at 100-1000 M concentrations of mercuric chloride, sodium arsenate and copper sulphate. While no statistically significant differences in colony diameters of P. lilacinus were observed at 100-1000 M concentrations of lead nitrate, significantly increased colony diameters were recorded during 52-184 hrs of incubation at all concentrations (100 and 1000 M) of lead nitrate compared to 151 controls. The colony diameter of P. lilacinus and V. chlamydosporium were measured in presence of a fungicide, Carbendazim, at different concentrations. Significant inhibition in colony diameters of P. lilacinus and V. chlamydosporium was observed at all concentrations (0.5-4.5 ppm) of carbendazim compared to controls. Full grown plates of P. lilacinus and V. chlamydosporium baited with viable eggs of Fasciola gigantica and Gigantocotyle explanatum revealed that V. chlamydosporium to be more vigorous in its egg parasitic ability compared to P. lilacinus. Distortion of eggs started on day 2-3 of egg baiting in CMA plates of V. chlamydosporium with complete distortion by day 7. On the contrary, P. lilacinus exhibited very limited egg parasitic ability. Even some of the baited eggs showed development of miracidia inside. Ten oligonucleotide primers (10-mer) of arbitrary sequence revealed a total of 146 scorable amplicons. Distinct fingerprints between the fungi were obtained by most of the primers. The sequence information obtained from the unique RAPD fragments of each of the fungus was submitted in the Genebank for genebank accession number. On Blast Search, sequence information obtained from V. chlamydosporium scored 98% similarity with Aspergillus niger (NW_001594280). Comparison of these two sequence information revealed 3.55% nucleotide sequence variation. Similarly, RAPD fragment generated by P. lilacinus (EF569593) scored 96% similarity on Blast Search with Aspergillus nidulans FGSC A4, hypothetical protein (XM_656131). Sequence information of the RAPD fragments generated by D. flagrans and A. oligospora (EF569594 and EF569595, respectively) did not match with any of the reference sequence information in the public database or Genebank. Primer set designed to amplify the specific fragment of V. chlamydosporium produced the predicted fragment size (310bp) without giving any amplification in other fungi. Primer set designed for P. lilacinus amplified the predicted fragment (600bp). It also produced one amplicon for V. chlamydosporium but of different fragment size. Primer set designed for D. flagrans produced the specific fragment of predicted size without giving any amplification in other fungi.
 
Date 2017-01-28T11:13:38Z
2017-01-28T11:13:38Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/97631
 
Language en_US
 
Format application/pdf
 
Publisher Chhattisgarh Kamdhenu Vishwavidyalaya, Durg