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Improvement of rhizobial inoculants for mungbean (Vigna radiata (L.)Taub.) crop

KrishiKosh

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Title Improvement of rhizobial inoculants for mungbean (Vigna radiata (L.)Taub.) crop
 
Creator Pawaria, Shikha
 
Contributor Gera, Rajesh
 
Subject Planting, Bacteria, Biological development, Productivity, Root nodulation, Nitrogen, Application methods, Rhizobium, Iaa, Biological phenomena
 
Description Legumes are among the World's most important crops. The family Fabaceae comprises of
around 20,000 plant species in 650 different genera. Mungbean belongs to the Papilionoideae family,
order Leguminosae and botanically recognized as Vigna radiata (L.). As a leguminous plant,
mungbean could be nodulated by rhizobia, causing the formation of a new organ nodule and establish a
nitrogen-fixing symbiosis. So the enhancement of biological nitrogen fixation and crop productivity,
rhizobial inoculants have been considered to be of prime importance. PGPR can improve the quality of
plant growth directly by nitrogen fixation and indirectly by producing siderophore, ammonia, IAA and
bacteriocin. The use of PGPR offers an attractive way to replace chemical fertilizers, pesticides and
supplements. In the present investigation, a total of 54 new mungbean rhizobial isolates were isolated
from healthy pink nodules of mungbean plant and 76 mungbean rhizobial isolates already existing in
the departmental culture collection, obtained from different districts of Haryana and Rajasthan state.
Out of all the tested rhizobial isolates 50% showed nitrogenase activity, which ranged between 50-355
n moles ethylene h-1 mg -1 protein. P-solubilization activity of rhizobial isolates on solid as well as
liquid medium was restricted to 50% of the isolates only and their P-solubilization index (P-SI) varied
from 1.0 to 3.9. Majority of rhizobial isolates were found to be IAA producers with production range
(1.00-55.20 μg/ml). Most of the isolates were also able to excrete ammonia, which varied from 1.09 to
4.89 μg/ml. Only 44 rhizobial isolates were found to produce bacteriocin against a sensitive strain of
mungbean, MS 58 by forming a clear zone of inhibition. Siderophore production was observed in 72%
of the isolates. Five promising rhizobial isolates namely MR 63, MR 54, MH 8b2, MH 6c1 and MB
17a, which showed 4 to 6 traits were selected for nodulation efficiency and plant growth parameters
under pot house conditions. Nodulation efficiency of isolate MB 17a and MH 8b2 was better on the
basis of nodule wt. after 45 and 60 days of sowing, while the isolate MR 63 and MH 8b2 showed
higher plant biomass after 60 of sowing, when compared with reference strains of rhizobium 703 which
is inoculated either individually or in co-inoculation with PSB.
 
Date 2016-10-05T09:13:25Z
2016-10-05T09:13:25Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/79940
 
Language en
 
Format application/pdf
 
Publisher CCSHAU