Record Details

Studies on genetic variability in male fertility restorer lines of sunflower (Helianthus annuus L. ) using molecular markers

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Studies on genetic variability in male fertility restorer lines of sunflower (Helianthus annuus L. ) using molecular markers
 
Creator Neyol, Esha
 
Contributor Boora, K.S.
 
Subject Genetic variability, Helianthus annuus, Molecular markers, Male fertility restoration
 
Description Sunflower is currently one of the most important oil producing crops in large
parts of the worlds. Cytoplasmic Male Sterility (CMS) and genetic fertility restoration
have been used for commercial production of hybrids. The present study was
conducted to estimate genetic variability among thirty three male fertility restorer lines
of sunflower. DNA extraction protocol and PCR amplification condition were
standardized with required modifications. As a result, the highest quantity of DNA was
extracted form AK-l-R (2545μg/ml) whereas the genotypes RHA-298 yielded lowest
quantity of DNA (6825μg/ml). Agarose gel electro-phoresis and U.V.
spectropholometer were used to test quality of DNA. The DNA samples were of good
quality and the ratio of absorbance ranged from 1.74-1.96.
For the optimum amplification of DNA in PCR 100ng genomic DNA, 3U of
Taq DNA Poly., 1.5mM MgCl2, 1mM dNTPs, 1ml of 10X Taq DNA poly reaction
buffer, 10um of primer and 400C annealing temperature were used. Out of thirty
randum primers screened, twenty two produced amplification while eight produced no
amplification. All twenty two premiers showed polymorphism among different
genotypes. A total of sixty four amplified products were obtained and all were
polymorphic. For all the genotypes evaluated, 1-6 bands were obtained with an average
of 2.90 per primer.
All the genotypes were distinguishable with combination of polymorphic bands
produced by various primers. Estimates of genetic similarity based on Nei and Li
equation ranged from five per cent to forty seven per cent indicating very high genetic
variability among the male fertility restorer lines of sunflower. Based on cluster analysis
using UPGMA, all genotypes could be divided into tow clusters. One cluster contained
two genotypes (HB-375 and NDR-1) and second cluster had rest thirty one lines.
Second cluster was further divided into two sub-clusters, comprising eighteen and
thirteen genotypes each. These results indicate that RAPDs can be used for
identification and discrimination of male fertility restorer lines of sunflower. These
highly diverse lines may be further used for production of hybrids of sunflower.
 
Date 2016-11-29T14:52:43Z
2016-11-29T14:52:43Z
2005
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/88165
 
Language en
 
Format application/pdf
 
Publisher CCSHAU