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Genetic screening and characterization of Type III effector genes encoding Xanthomonas outer protein (Xop) in Xanthomonas axonopodis pv. punicae causing bacterial blight of pomegranate

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Title Genetic screening and characterization of Type III effector genes encoding Xanthomonas outer protein (Xop) in Xanthomonas axonopodis pv. punicae causing bacterial blight of pomegranate
M Sc
 
Creator RISHIKESH KUMAR
 
Contributor K. K. Mondal
 
Subject Xanthomonas axonopodis pv. punicae, bacterial blight, effector, Xanthomonas outer protein, null mutant
 
Description T-8696
Bacterial blight caused by Xanthomonas axonopodis pv. punicae is an important
disease of pomegranate. This study documented the presence of six Xop type T3SS
effectors, namely XopC2, XopE1, XopL, XopN, XopQ and XopZ in X. axonopodis pv.
punicae using gene specific PCR. One major effector, XopN was studied in detailed to
understand its possible role in bacterial virulence. Overlapping extension PCR was
employed to generate a null mutant (Xap ΔxopN) for XopN of strain ITCCBD0003 of X.
axonopodis pv. punicae (Rif100). Kanamycin gene was used to replace the target gene. It
was observed that on NA and YP agar media the mutant took 96-120 h to become visible
colonies, while the wild type took only 36-48 to appear in visible size. This observation
supported the fact that deletion of XopN slowed down the growth rate of the blight
pathogen. The absence of intense watersoaking on infiltrated pomegranate leaf with
XopN-mutant (Xap ΔxopN) of X. axonopodis pv. punicae further confirmed the role of
the XopN effector in contributing virulence to the host bacterium. The population count
of Xap ΔxopN as well as wild strains after infiltration indicated 20.22% reduction in
growth of the mutant over wild strain suggesting that multiple effectors play role in
governing virulence to the bacterial pathogen. The present study concluded that XopN
like other phytopathogenic bacteria, play important role in governing virulence to the
host bacterial pathogen; and the loss of it in the mutant Xap ΔxopN could able to suppress
the PAMP-trigged immunity only partially, not completely.
 
Date 2016-09-06T19:54:40Z
2016-09-06T19:54:40Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/75757
 
Format application/pdf
 
Publisher IARI, Division of Plant Pathology