MOLECULAR CHARACTERIZATION OF CITRUS TRISTEZA VIRUS ISOLATES FROM DIFFERENT GEOGRAPHICAL REGIONS OF INDIA
KrishiKosh
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Title |
MOLECULAR CHARACTERIZATION OF CITRUS TRISTEZA VIRUS ISOLATES FROM DIFFERENT GEOGRAPHICAL REGIONS OF INDIA
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Creator |
SUSHEEL KUMAR
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Contributor |
Kajal Kumar Biswas
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Subject |
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Description |
t-8260
Citrus tristeza virus (CTV), a member of genus Closterovirus (family Closteroviridae), is one of the most destructive pathogens of citrus in most of the citrus growing countries worldwide including India. It is phloem limited, aphid transmitted, long flexuous virus with single stranded positive sense RNA genome. Citrus samples from nine different citrus orchards of Indian Agricultural Research Institute, New Delhi experimental farm were tested by DAC-ELISA and RT-PCR. The orchards of sweet orange (Citrus sinensis) were found to be CTV infected with estimated disease incidence of 46 to 57%. The orchards of Kinnow mandarin (C. reticulata), Kagzi kalan (C. lemon) and Pumello (C. paradisi) were not found to be infected. One CTV isolate D1 from sweet orange field was biologically indexed using graft inoculation, and found to infect Kagzi lime (C. aurantifolia), sweet orange, Darjeeling mandarin (C. reticulata) and sour orange (C. aurantium). Three CTV isolates D1, D7 and D15 from Delhi were characterized at molecular level based on amplification, molecular cloning and sequencing of 5´ORF1a fragment (404 nt) of CTV genome. The sequence of Delhi isolates were analyzed, and compared with other CTV isolates. All the Delhi isolates showed nucleotide identities from 85 to 92% among them, and phylogenetic analysis classified the Delhi isolates into three distinct groups, indicating occurrence of extensive genetic diversity in CTV in Delhi region. |
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Date |
2016-09-30T16:18:22Z
2016-09-30T16:18:22Z 2010 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/79653
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Format |
application/pdf
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Publisher |
iari, Division of Plant Pathology
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