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MOLECULAR CHARACTERIZATION OF CITRUS TRISTEZA VIRUS ISOLATES FROM DIFFERENT GEOGRAPHICAL REGIONS OF INDIA

KrishiKosh

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Title MOLECULAR CHARACTERIZATION OF CITRUS TRISTEZA VIRUS ISOLATES FROM DIFFERENT GEOGRAPHICAL REGIONS OF INDIA
M Sc
 
Creator SUSHEEL KUMAR
 
Contributor Kajal Kumar Biswas
 
Subject ---
 
Description t-8260
Citrus tristeza virus (CTV), a member of genus Closterovirus (family Closteroviridae), is
one of the most destructive pathogens of citrus in most of the citrus growing countries
worldwide including India. It is phloem limited, aphid transmitted, long flexuous virus
with single stranded positive sense RNA genome. Citrus samples from nine different
citrus orchards of Indian Agricultural Research Institute, New Delhi experimental farm
were tested by DAC-ELISA and RT-PCR. The orchards of sweet orange (Citrus sinensis)
were found to be CTV infected with estimated disease incidence of 46 to 57%. The
orchards of Kinnow mandarin (C. reticulata), Kagzi kalan (C. lemon) and Pumello (C.
paradisi) were not found to be infected. One CTV isolate D1 from sweet orange field was
biologically indexed using graft inoculation, and found to infect Kagzi lime (C.
aurantifolia), sweet orange, Darjeeling mandarin (C. reticulata) and sour orange (C.
aurantium). Three CTV isolates D1, D7 and D15 from Delhi were characterized at
molecular level based on amplification, molecular cloning and sequencing of 5´ORF1a
fragment (404 nt) of CTV genome. The sequence of Delhi isolates were analyzed, and
compared with other CTV isolates. All the Delhi isolates showed nucleotide identities
from 85 to 92% among them, and phylogenetic analysis classified the Delhi isolates into
three distinct groups, indicating occurrence of extensive genetic diversity in CTV in
Delhi region.
 
Date 2016-09-30T16:18:22Z
2016-09-30T16:18:22Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/79653
 
Format application/pdf
 
Publisher iari, Division of Plant Pathology