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Analysis of diversity for micronutrient content and DNA fingerprinting in Greengram [Vigna radiata (L.) Wilczek]

KrishiKosh

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Title Analysis of diversity for micronutrient content and DNA fingerprinting in Greengram [Vigna radiata (L.) Wilczek]
 
Creator Jyoti
 
Contributor Yadav, Neelam
 
Subject Coffee, Costs, Productivity, Marketing, Manpower, Economics, Alternative agriculture, Tillage equipment, Irrigation, Fertilizers
 
Description Vigna radiata (L.) Wilczek, commonly called mungbean or
greengram is the third most important pulse crop of India occupying
nearly 3 million hectare. Mungbean provides inexpensive, low flatulence
and easily digestible protein available in several edible forms that
complements staple rice diet in Asia. India is the primary greengram
producer and contributes about 75% of world production. Iron and Zinc
are important micronutrients for human health whose deficiency causes
anemia and malfunctioning of immune system. Commercial cultivars
contain low levels of iron and zinc and it is important to assess genetic
-IIvariability
in available germplasm for improving micronutrient content in
commercial cultivars. The present study was undertaken to study RAPD
polymorphism among 16 mungbean genotypes using 33 decamer primers
in PCR reaction. A total of 148 amplified bands were produced out of
which 142 were polymorphic and 6 were monomorphic. For the
genotypes studied, upto bands were produced with an average of 6.16
bands per primer. The size of amplified bands ranged from 150-3000 bp.
Some unique bands were also produced, which could be used to
distinguish these genotypes. The similarity coefficients between different
genotypes ranged from 0.40-0.89 with an average similarity value of
0.65. At an arbitrary cut-off at 60 per cent similarity level on a
dendrogram, the mungbean accessions were categorized into two major
clusters. Satya and Asha were found to genetically similar. ML776
showed high iron and zinc content while Satya was poor in iron as well as
zinc content. OPB7 primer showed 900 bp amplified product in ML776
that was found absent in Satya. These two genotypes can be used in
developing population for linkage mapping. The results indicated that
RAPD markers were efficient for identification of Vigna radiata
genotypes and for determination of the genetic relationships among them.
 
Date 2016-11-16T09:57:55Z
2016-11-16T09:57:55Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85773
 
Language en
 
Format application/pdf