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ISOLATION AND MORPHOLOGICAL CHARACTERIZATION OF USTILAGINOIDEA VIRENS, CAUSING FALSE SMUT OF RICE

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Title ISOLATION AND MORPHOLOGICAL CHARACTERIZATION OF USTILAGINOIDEA VIRENS, CAUSING FALSE SMUT OF RICE
M Sc
 
Creator MATHEW SEIKHOLEN BAITE
 
Contributor Raj Kumar Sharma
 
Subject Ustilaginoidea virens; isolation; cultural characters; ITS sequencing.
 
Description T-8695
False smut of rice, caused by the fungus, Ustilaginoidea virens, once
considered a minor disease, is now an emerging disease of rice causing havoc in many
rice growing areas of the world. The isolation technique of Ustilaginoidea virens has
been standardized. Surface sterilization of smut balls with 1% sodium hypochlorite
solution followed by 70% ethanol wash yielded better results than 0.1% mercuric
chloride solution. Best growth of the fungus was achieved on potato sucrose agar
medium (pH 6.0) at 27°C, where the mean colony diameter of different isolates varied
from 25 to 40 mm. Incubation in dark was found highly conducive for the growth of
the fungus. Ustilaginoidea virens in culture medium showed creamy white colony,
which was flat or raised with slight undulations, mycelium was fluffy, compact and
leathery. Chlamydospores are formed at the centre or margin of the colony, which
appears orange or yellowish and later greenish. The isolated fungus was identified
according to the cultural descriptions given by Sharma and Joshi (1975) and Verma
and Singh (1988). The identity of the fungal pathogen of rice false smut was also
confirmed through ITS sequencing which showed 98-99 per cent identity with
Ustilaginoidea virens in NCBI-BLAST analysis. The perfect isolation technique
coupled with information on optimization of culture conditions of Ustilaginoidea
virens will immensely contribute to further studies on the fungus and the disease it
caused in India.
 
Date 2016-09-06T19:57:27Z
2016-09-06T19:57:27Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/75758
 
Format application/pdf
 
Publisher IARI, Division of Plant Pathology,