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Studies on bacterial parasite, pasteuria penetrans (Ex Thorne) sayre and starr infecting rice rootknot nematode, meloidogyne graminicola golden and birchfield,1965

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Title Studies on bacterial parasite, pasteuria penetrans (Ex Thorne) sayre and starr infecting rice rootknot nematode, meloidogyne graminicola golden and birchfield,1965
 
Creator Thakur, Sharmishtha
 
Contributor Walia, R.K.
 
Subject Markets, Marketing, Economics, Vegetables, Millets, Sorghum, Costs, Biological phenomena, Productivity, Policies
 
Description North eastern districts of Haryana namely, Ambala, Kaithal, Karnal, Kurukshetra, Panchkula and
Yamunanagar were surveyed for incidence of indigenous isolate(s) of Pasteuria penetrans on
Meloidogyne gramincola. Out of 75 nursery sites of rice, M. graminicola was recorded in 53 per cent
samples (80% in bioassays). No samples collected from Haryana revealed the incidence of P.
penetrans. However, out of 15 populations of M. graminicola collected from different parts of India,
four populations namely, Bhubaneswar (Odisha), Kalyani (West Bengal), Jorhat (Assam) and Vellayani
(Kerala), showed the incidence of P. penetrans. Bhubaneswar isolate of P. penetrans (MgPp) was
selected for further studies. In the host range studies on MgPp, no endospore attachment was observed
on Heterodera avenae, H. cajani, H. zeae, Tylenchulus semipenetrans and Aphelenchoides sp. but spore
encumbrance was recorded on J2 of Meloidogyne javanica, M. incognita, besides M. graminicola.
MgPp completed its life cycle and spore-filled females were observed on all the three species of
Meloidogyne. Comparison of the life cycle of the two strains of P. penetrans, i.e., MgPp on M.
graminicola and another isolate (MjPp) on M. javanica, revealed that although M. graminicola adult
females were formed by 16th day, but mature endospores of MgPp were formed only by 24th day; while
M. javanica adult females were formed by 24th day and MjPp reached mature spore stage by 28th day.
The sequence of developmental stages of P. penetrans was similar in both the strains. The life cycles of
respective nematode species and P. penetrans strains were synchronized. Morphological comparison
was made on the spores of MgPp and MjPp by light microscopy and SEM. The endospore of MgPp
was larger compared to MjPp, however, sporangium was bigger in MjPp. SEM observations, however,
could not resolve any differences in the endospore as well as sporangium measurements. In one screenhouse
trial, MgPp spore suspension was applied as nursery soil treatment in three doses (1x103,
0.5x104, 1x104 spores per g soil). Higher doses of MgPp improved rice seedling growth. MgPp reduced
galling and nematode egg production at higher doses. Up to 43 per cent infection of nematode females
was recorded at 1x104 dose. In another screen-house trial, MgPp was applied as seed coat (1-4% w/w).
No significant differences were recorded among the treatments on plant growth. The impact of MgPp
in the reduction of galling was evident at doses of 2-4%. Reduction in egg production and female
infection was, however, recorded only at 4% dose.
 
Date 2016-10-05T15:38:57Z
2016-10-05T15:38:57Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/80054
 
Language en
 
Format application/pdf
 
Publisher CCSHAU