Selection and characterization of tomato calli tolerant to alternaria solani (Ellis and Martin) jones and grout
KrishiKosh
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Title |
Selection and characterization of tomato calli tolerant to alternaria solani (Ellis and Martin) jones and grout
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Creator |
Venkatesh C.
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Contributor |
Gandhi, S.K.
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Subject |
Apiculture, Honey, Biological phenomena, Audiovisual aids, Productivity, Economics, Animal population, Manpower, Selection, Imports
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Description |
Present study was carried out with the objective to investigate the callogenic response of tomato genotypes to culture filtrates of Alternaria solani and to characterize the calli showing resistance along with non-selected calli, based on few biochemical parameters. To study the callogenic response of tomato genotypes to A. solani, different concentration of culture filtrates were added in MS medium supplemented with 0.5 mg/l NAA & 3 mg/l BAP which was found most suitable for callus induction to all the four genotypes viz., H-121, sel- 7, DT-1, BL982. Percent reduction in callus formation in response to A. solani culture filtrates was more in highly susceptible genotypes BL982 & DT-1 than moderately resistant genotypes. In vitro screening with culture filtrates of early blight pathogen revealed a similar behavior with field reactions of the genotypes to the disease. Since twenty percent concentration of culture filtrates was lethal to all genotypes except for H-121, 15% concentration of culture filtrates can be used for in vitro screening for selection of resistant genotypes. Increase in concentration of culture filtrates resulted in decrease of fresh as well as dry weights of calli derived from all the genotypes. Total sugars, reducing sugars were high after selection with A. solani culture filtrates in calli of H-121, Sel-7 but highly susceptible genotypes showed reduced sugar contents. Levels of phenols & flavonols were high in all the genotypes after selection. Activity of all the three enzymes viz. polyphenol oxidase, peroxidase and catalase increased with response to increase in concentration of culture filtrates. |
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Date |
2016-11-24T10:07:41Z
2016-11-24T10:07:41Z 2007 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/87351
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Language |
en
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Format |
application/pdf
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Publisher |
CCSHAU
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