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Selection and characterization of tomato calli tolerant to alternaria solani (Ellis and Martin) jones and grout

KrishiKosh

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Title Selection and characterization of tomato calli tolerant to alternaria solani (Ellis and Martin) jones and grout
 
Creator Venkatesh C.
 
Contributor Gandhi, S.K.
 
Subject Apiculture, Honey, Biological phenomena, Audiovisual aids, Productivity, Economics, Animal population, Manpower, Selection, Imports
 
Description Present study was carried out with the objective to investigate
the callogenic response of tomato genotypes to culture filtrates of
Alternaria solani and to characterize the calli showing resistance along
with non-selected calli, based on few biochemical parameters. To
study the callogenic response of tomato genotypes to A. solani,
different concentration of culture filtrates were added in MS medium
supplemented with 0.5 mg/l NAA & 3 mg/l BAP which was found most
suitable for callus induction to all the four genotypes viz., H-121, sel-
7, DT-1, BL982. Percent reduction in callus formation in response to
A. solani culture filtrates was more in highly susceptible genotypes
BL982 & DT-1 than moderately resistant genotypes. In vitro screening
with culture filtrates of early blight pathogen revealed a similar
behavior with field reactions of the genotypes to the disease. Since
twenty percent concentration of culture filtrates was lethal to all
genotypes except for H-121, 15% concentration of culture filtrates can
be used for in vitro screening for selection of resistant genotypes.
Increase in concentration of culture filtrates resulted in decrease of
fresh as well as dry weights of calli derived from all the genotypes.
Total sugars, reducing sugars were high after selection with A. solani
culture filtrates in calli of H-121, Sel-7 but highly susceptible
genotypes showed reduced sugar contents. Levels of phenols &
flavonols were high in all the genotypes after selection. Activity of all
the three enzymes viz. polyphenol oxidase, peroxidase and catalase
increased with response to increase in concentration of culture
filtrates.
 
Date 2016-11-24T10:07:41Z
2016-11-24T10:07:41Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/87351
 
Language en
 
Format application/pdf
 
Publisher CCSHAU