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Morphological and molecular characterization of citrus germplasm

KrishiKosh

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Title Morphological and molecular characterization of citrus germplasm
 
Creator Laxman, Tele Ajinath
 
Contributor Sherawat, S.K.
 
Subject Fruits, Genotypes, DNA, Acidity, Rapd, Limes, Genetics, Confectionery, Polymorphism, Developmental stages
 
Description Sixteen citrus genotypes representing 7 species and two hybrids
(C.sinensis, C. reshini, C. lemon, C. jambhiri, C. aurantifolia, C. limettoides, C.
paradisi, C.nobilis X C.deliciosa, and C. sinensis X P. trifoliata) were analyzed
using 32 morphological characters and 10 RAPD markers. Out of 32
morphological traits studied, spine shape did not show any variation, while
the remaining traits showed variability to considerable extent. With respect to
RAPD analysis, a total of 82 clear and reproducible bands were obtained. All
the bands were found to be polymorphic since no primer showed
monomorphism. For the genotypes tested, between 4-12 bands were obtained
for each primer with an average of 8.2 bands per primer. Morphological
observations of different genotypes were used to calculate pair wise
dissimilarity index. Similarly, RAPD data was also used to calculate similarity
matrix which ranged from 0.29 to 0.89 with an average similarity of 0.68
across all the genotypes. The dendrogram constructed with morphological
results divided the 16 genotypes in to five groups. First cluster comprised of
eight genotypes; second and third cluster comprised of single genotype each
viz., Bhadri lemon and Pant lemon, respectively; fourth cluster comprised of
all grpefruit cultivars along with Sweet lime and fifth cluster comprised of two
genotypes Cleopatra and Acid lime. Bhadri lemon and Pant lemon showed
highest dissimilarity followed by Bhadri Lemon and Acid lime. On the other
hand, least dissimilarity was observed between the genotypes Kinnow and
Mosambi. While that of the constructed with RAPD markers clustered into
three groups. The first cluster consisted of Pineapple and Cleopatra at
similarity coefficient of 0.89. Mosambi and Blood Red, Citrange hybrid
(Troyer) and Sweet lime clustered under the second major group, whereas
third major cluster comprised of remaining all the genotypes. Cluster
obtained from morphological characterization was only partially correlated
with with the clusters obtained from molecular characterization. Though
these accessions represent an important source of genetic diversity in citrus
and can be used in future breeding programs.
 
Date 2016-11-16T11:46:19Z
2016-11-16T11:46:19Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85805
 
Language en
 
Format application/pdf
 
Publisher CCSHAU