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EXPRESSION PROFILING OF LEAF RUST RESISTANCE (Lr) GENE IN WHEAT (Triticum aestivum L.)

KrishiKosh

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Title EXPRESSION PROFILING OF LEAF RUST RESISTANCE (Lr) GENE IN WHEAT (Triticum aestivum L.)
 
Creator SHAILENDRA KUMAR JHA
 
Contributor K. V. Prabhu
 
Subject ---
 
Description T-8521
Spring wheat variety HD 2329 and its near isogenic line (NIL) HD 2329+Lr28 were
challenge inoculated with leaf rust pathogen Puccinia triticina race 77-5 (Syn. 121R63-1) to
identify the genes differentially expressed during host pathogen interaction (HPI). Differential
display reverse transcription (DDRT)-PCR and Real-time PCR techniques were explored to
identify the transcripts and verify the expression level. In total 17 differentially expressed
transcripts were identified in the study, out of which 8 (DDTR 1-8) were from incompatible
interaction leading to resistance and 9 (DDTS 1-9) were from compatible interaction leading
to susceptibility. DDTR 1, DDTR 2, DDTS 1, DDTS 6, DDTS 8 and DDTS 9 were unique
transcripts which did not match with sequences available in the database. Sequences of six
incompatibility specific transcripts were highly similar to the sequences available in database
for the biotic stress, abiotic stress and developmental stage specific ESTs in plants while, 5
compatibility specific transcripts showed high homology with sequences available on stage
specific ESTs of P. triticina. Real-time PCR study revealed specific amplification for DDTS
6 and DDTS 9 in the compatible interaction. Along with these transcripts, expression study of
some of the known signalling molecules like TaMAP Kinase, transcription factors (TF)
TaNAC8 and TabZIP1 were also performed. In all the 3 signalling molecules, level of
expression was up-regulated, but the level of up-regulation for TaMAP Kinase was higher in
the incompatible interaction than the compatible interaction. The TFs TaNAC 8 and TabZIP1
showed higher expression in the compatible interaction. The homology and expression
patterns of transcripts identified during present study showed crosstalk between signalling
pathways of biotic stress, abiotic stresses and plant development. Thus, the transcripts
identified and differentially expressed during wheat and leaf rust interaction will be helpful in
manipulating the pathways involved during appearance of resistance in host and emergence of
virulence in pathogen.
 
Date 2016-12-01T15:12:47Z
2016-12-01T15:12:47Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/88704
 
Format application/pdf
 
Publisher IARI, DIVISION OF GENETICS