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Identification of resistance sources against Soybean mosaic virus in soybean (Glycine max (L.)Merr.2n=40).

KrishiKosh

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Title Identification of resistance sources against Soybean mosaic virus in soybean (Glycine max (L.)Merr.2n=40).
M Sc
 
Creator Deepika Cheruku
 
Contributor S. K. Lal
 
Subject soybeans, sowing, biological phenomena, planting, viruses, genotypes, genes, application methods, diseases, land resources
 
Description T-8654
A set of 42 genotypes were selected for the study. Infection index for soybean mosaic virus was determined under natural incidence. On the basis of natural incidence of SMV, infection index varied between 5.5 to 100%. Out of 42 genotypes tested four genotypes namely SL 900 (5.5%), PS 1505 (5.5%), SL 958 (11.1%) and DS 2706 (11.1%) were found to be HR whereas Bragg (29.6%) showed moderately resistant (MR) reaction. All other genotypes were found to be susceptible to SMV under natural incidence. Out of 42 genotypes, 28 genotypes showing different levels of resistance against SMV under natural incidence were subjected to mechanical inoculation. The results of the challenge inoculation did not match the results of the field screening. All the lines showing HR reaction were confirmed to be HR in the challenge inoculation test (OD value of ELISA at 405nm 0.342). Out of 23 genotypes which were found to be susceptible under field screening only seven were found to be susceptible under challenge inoculation method (OD value of ELISA at 405nm < 0.342). The polymorphism among twenty eight genotypes for SSR markers linked to Rsv1 gene (Satt 114) and Rsv4 gene (Satt 542) was also surveyed. There was high level of polymorphism for Satt 114. However there was very less variation for Satt 542. Genotypes DS 2706 and SL 900 were identified as resistant sources for SMV. The resistance was confirmed in all the three studies i.e., screening through natural incidence, challenge inoculation and presence of Satt 114 marker which is linked to Rsv1 gene. The results of the present study can be used for selecting contrasting parent for development of mapping population or as a source for resistance in the breeding programme.
 
Date 2016-09-17T16:58:31Z
2016-09-17T16:58:31Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/77244
 
Format application/pdf
 
Publisher IARI, DIVISION OF GENETICS