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Plant regeneration studies in female papaya (Carica papaya L.)

KrishiKosh

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Title Plant regeneration studies in female papaya (Carica papaya L.)
 
Creator Renu Singh
 
Contributor Kharb, Pushpa
 
Subject Micropropagation, Carica papaya L.
 
Description The present study was carried out on Selection-I cultivars of papaya (Carica
papaya L.) by using shoot tip and axillary bud as explants and media supplementation
with the main aim to assess the feasibility for clonal propagation in female plants. The
study comprised of regeneration of explants in the establishment media, proliferation
of regenerated shoots, rooting of regenerated plantlets and transfer of rooted plantlets
in soil (pots). Total of 29 media were used for shoot regeneration. Based on the results
of this study shoot tip explant gave the highest shoot induction response (70%) on
RB9 (MS+BAP (2.0 mg l-1) + NAA (0.1 mg l-1) medium while axillary bud explant
gave the highest shoot induction response on RB2 (MS + BAP (1.0 mg l-1) and RB9
(MS + BAP (2.0 mg l-1) + NAA (0.1 mg l-1) media giving 63 to 70% response. Thus,
RB9 (MS + BAP (2.0 mg l-1) + NAA (0.1 mgl-1) medium was found to be the best
establishment medium.
Maximum rooting percentage (67%) was exhibited by RR6 medium
(MS + IBA (2.0 mg l-1). The plantlets transplanted in potting mixture composed of
sand + soils + FYM in equal proportion exhibited maximum survived (70%). Highest
percentages of regeneration from shoot tip explants. Shoot tip explants were found to
be the better explant. Effect of silver nitrate (AgNO3) was studied and no enhancing
effect of AgNO3 was observed in case of both the explants (Shoot tip and axillary
bud).
 
Date 2016-11-29T14:57:42Z
2016-11-29T14:57:42Z
2005
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/88168
 
Language en
 
Format application/pdf
 
Publisher CCSHAU