Studies on the insecticidal properties of datura metel against helicoverpa armigera (Hubner)
KrishiKosh
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Title |
Studies on the insecticidal properties of datura metel against helicoverpa armigera (Hubner)
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Creator |
Virender Singh
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Contributor |
Ram Singh
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Subject |
Datura, Helicoverpa armigera, Fractions, Extracts
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Description |
The studies on the insecticidal properties of Datura metel leaves and seeds extract in methanol and its fractions in hexane, benzene, chloroform and acetone were conducted under laboratory conditions against Helicoverpa armigera (Hubner). Treatments included 2.5, 5.0, 7.5, 10.0 per cent concentrations of methanol extract and 0.5, 1.0, 1.5, 2.0 per cent concentrations of fractions to compare the results with water/acetone as control. Methanol extracts (2.5,5.0,7.5,10.0%) of leaves and seeds of Datura metel and their fractions (0.5,1.0,1.5,2.0%) in benzene, acetone, chloroform and hexane were evaluated against Helicoverpa armigera (Hubner). Leaf extracts and fractions treated food showed significant effect on larval mortality (12.22-74.43%), larval period (12.00-18.33 days), larval weight (303-495 mg), pupation (30.38-87.31%), pupal period (9-11.33 days) and adult emergence (28.33-86.95%). Almost similar results were obtained with the extracts and fractions of seeds. In contact toxicity experiment, first instar larval mortality ranged from 26.66% (2.5% methanol extract) to 76.66% (2.0% benzene fraction) in leaf extract and fractions. Corresponding mortality in seeds extracts and fractions was 30.0 to 80.0%. Leaf extract and fractions also caused significant effect on CI (3.41-8.58), GR (0.14-0.35mg/day), ECI (2.57-7.52%), AD/AE (87.65-98.06%) and ECD (2.62-9.81%). Similar results were obtained with Datura seeds. Antifeedancy due to extract and fractions of leaves ranged from 5.08% in 0.5% benzene fraction to 39.46% in 2% acetone fraction. In Datura seeds it ranged from 7.01% in 0.5% hexane fraction to 41.15% in 2% acetone fraction. Under oviposition studies, adults were allowed to lay eggs on oviposition substrate under choice, no choice and without actual contact situations. Minimum eggs were laid in 10% methanol extract treatment while maximum were laid in benzene fraction 0.5%. Effects of leaves and seeds were almost similar. When the adults were allowed to feed on sucrose diet containing different concentrations of extract and fractions of Datura leaves, number of eggs laid ranged from 151(2% chloroform fraction) to 241 (0.5% hexane fraction) and hatching ranged from 64.88% (2% hexane fraction) to 75.75% (0.5% acetone fraction). Similar results were obtained with seed extract and fractions. Egg hatching (45.73-84.16%) was also affected significantly when eggs were directly treated with extract and fractions of Datura leaves and seeds. |
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Date |
2016-11-30T13:27:32Z
2016-11-30T13:27:32Z 2005 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/88383
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Language |
en
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Format |
application/pdf
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Publisher |
CCSHAU
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