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Selection and characterization of rapeseed and mustard calli tolerant to sclerotinia sclerotiorum (Lib.) de bary

KrishiKosh

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Title Selection and characterization of rapeseed and mustard calli tolerant to sclerotinia sclerotiorum (Lib.) de bary
 
Creator Watpade, Santosh Ganpat
 
Contributor Mehta, Naresh
 
Subject Fungi, Diseases, Planting, Biological phenomena, Concentrates, Enzymes, Acidity, Organic acid salts, Organic acids, Sclerotinia sclerotiorum
 
Description Present study was carried out with the objective to investigate
the callogenic response of rapeseed-mustard species to culture filtrate
of Sclerotinia sclerotiorum, oxalic acid and to characterize the calli
showing tolerance to culture filtrate along with non-selected calli,
based on few biochemical parameters. To study the callogenic response
of rapeseed-mustard species to S. sclerotiorum, different concentrations
of culture filtrate and oxalic acid were added in MS medium
supplemented with NAA 1 mg-1+ BAP 1 mg-1. NAA 1 mg-1+ BAP 1 mg-1
was found most suitable for callus induction to all the four species viz.,
B. juncea cv. RH-30, B. alba cv. Local, B. napus cv. GSH-1 and B.
carinata cv. HC-9002. Per cent callus formation in response to S.
sclerotiorum culture filtrate and oxalic acid was more in B. carinata cv.
HC-9002 than other Brassica species. Reduction in shoot length and
total seedling length due to presence of oxalic acid in germination
media was also minimum in B. carinata cv. HC-9002 than other
Brassica species. In vitro callus screening with S. sclerotiorum culture
filtrate and oxalic acid revealed a similar behavior with field reactions
IV
of the Brassica species to the disease. As well as seedling screening on
germination media amended with oxalic acid also showed correlation
with field reactions. Since twenty per cent concentration of culture
filtrate was lethal to some genotypes except B. napus cv. GSH-1 and B.
carinata cv. HC-9002 and 10mM concentration of oxalic acid is lethal
for all species, 15% concentration of culture filtrate and 5mM
concentration of oxalic acid can be used for in vitro screening for
selection of tolerant species. Increase in concentration of culture
filtrate and oxalic acid resulted in decrease of fresh as well as dry
weights of calli derived from all the species. Oxalic acid also caused
reduction in shoot length, root length and total seedling length of
rapeseed-mustard seedlings, 10 mM oxalic acid concentration can be
used for in vitro seedling screening and shoot length was better
parameter of susceptibility. Total soluble sugars and reducing sugars
were high after selection with S. sclerotiorum culture filtrate in calli of
B. napus cv. GSH-1 and B. carinata cv. HC-9002 but other species
showed less sugar contents. Level of phenol and flavanol were high in
all the species after selection. Activity of all the three enzymes viz.,
polyphenol oxidase, peroxidase and catalase increased with response to
increase in concentration of culture filtrate.
 
Date 2016-11-16T09:41:10Z
2016-11-16T09:41:10Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85770
 
Language en
 
Format application/pdf
 
Publisher CCSHAU