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Morphological and molecular characterization of chrysanthemum germplasm

KrishiKosh

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Title Morphological and molecular characterization of chrysanthemum germplasm
 
Creator Jai Kishan
 
Contributor Sehrawat, S.K.
 
Subject Genotypes, Developmental stages, Chrysanthemum, Fruits, Planting, Dna, Genetics, Polymorphism, Precipitation, Diseases
 
Description The study was undertaken with the objective to characterise chrysanthemum
genotypes by using morphological and molecular approaches. Morphologicaly all genotypes
showed significant variation. Amongst various genotypes, the maximum plant height (66.97
cm), plant spread (40.83 cm) and number of branches (54.80) was recorded in Maghi White.
The earliest flowering was observed in Ajay (54.10 days) while Sweta Singar recorded
longest duration of flowering (116.77 days). The highest number of flowers (219.53) and
yield per plant (562.37 g) were recorded in Sadbhavna and Maghi White respectively. Out of
thirty five ISSR primers, twenty eight ISSR primers showed amplification. Two sixty four
alleles were found with a mean of 9.43 alleles per locus. ISSR primers generated a high level
of average percentage of polymorphism i.e. 96.97%. The primers yielded average 9.43 bands
per primer. Overall size of PCR amplified products ranged between 120 bp and 1500 bp.
Dendrogram generated by ISSR markers separated genotypes into two major clusters which
were further divided into sub-clusters. The study revealed that ISSR molecular markers can
be used to assess polymorphism among the genotypes of chrysanthemum and can be useful
tool to supplement the distinctness, uniformity and stability analysis for plant variety
protection in future.
 
Date 2016-10-26T14:23:48Z
2016-10-26T14:23:48Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/82184
 
Language en
 
Format application/pdf
 
Publisher CCSHAU