ICAR Research Data Repository for Knowledge Management
Development of mutants of rhizobium spp. (Guar) with enhanced pectinolytic activity and competitive ability
KrishiKosh
View Archive Info| Field | Value | |
| Title |
Development of mutants of rhizobium spp. (Guar) with enhanced pectinolytic activity and competitive ability
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| Creator |
Sharma, Pankaj
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| Contributor |
Sikka, V.K.
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| Subject |
Cyamopsis tetragonoloba, Competitive ability, GFP transformation, Mutagenesis, Nodulation efficiency, Nitrogen fixation
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| Description |
Rhizobium spp. clusterbean (Guar) strains isolated from diverse locations having different antibiotic resistance pattern, growth kinetics on different media and plasmid profiles have been mutagenically improved for higher symbiotic infectivity. Five tested strains BcR-7, BcR-33, BcR-34, BcR-35 and BcR-40 with StmR marker were mutagenically treated by -irradiation and mutants were screened for their pectin degrading ability. Only ten upward mutants (which were showing better pectin degrading ability as compared to their parent strains) were further characterized and tested for their symbiotic infection ability and pectin utilizing ability. Additionally, competitive ability of parent strains and their mutants was evaluated by conjugally transferring GFP marker. Plant infection test with the parent strains and mutants gave 2. 4 folds increase competitive ability as evidenced by higher number of nodules produced on guar by mutants generated in this study. |
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| Date |
2016-12-01T10:03:56Z
2016-12-01T10:03:56Z 2005 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/88516
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| Language |
en
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| Format |
application/pdf
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| Publisher |
CCSHAU
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