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Studies on genetic diversity in cotton for cotton leaf curl virus disease by using RAPD technique

KrishiKosh

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Title Studies on genetic diversity in cotton for cotton leaf curl virus disease by using RAPD technique
 
Creator Minakshi
 
Contributor Luthra, O.P.
 
Subject Cotton, Deseases, Dna, Genotypes, Rapd, Genetics, Polymorphism, Planting, Biological phenomena, Pcr
 
Description Cotton is highly priced and economically important crop in the world
production and trade. Cotton leaf curl virus (CLCuV) disease is a major threat
to cotton production. Therefore, this study was conducted to prepare DNA
fingerprint database of 15 cotton genotypes and to assess genetic diversity
among them by using random amplified polymorphic DNA (RAPD) primers.
Fifteen RAPD primers were used to assess molecular polymorphism in 15
cotton genotypes. A total 135 amplified products were observed of which 16
were monomorphic and 119 polymorphic. Average polymorphism across the 15
genotypes was 87.5 per cent and a significant correlation (0.93, p < 0.01) was
observed between total number of bands and number of polymorphic bands.
Some of the primers gave unique bands for specific genotypes which further
can help in identification of genotypes. Fifteen genotypes were grouped into
two main groups based on polymorphism profile generated by using
NTSYS-PC programme. One is of resistant genotypes (exception H-1098, a
susceptible genotype) and other group is of susceptible genotypes at a similarity
coefficient of value 0.555. These two major groups further divided into various
subgroups according to similarity between them. HS-182 (S) and HS-6 (S) were
most diverse at a similarity coefficient of value 0.510 and 0.520, respectively,
merged with other susceptible and resistant genotypes at a similarity coefficient
of 0.555. Laxmi (R) and Laxmi (R) x LRA-5166 (R) (F1 hybrid) showed
maximum similarity i.e. 85 per cent and HS-182 (S) and HS-6 (S) x H-1098 (S)
(F1 hybrid) showed minimum similarity i.e. 40 per cent. Thus, RAPD primers
detected high level of polymorphism and fingerprint data generated in this
study can be used to improve the resistance among cotton varieties for cotton
leaf curl virus disease.
 
Date 2016-11-23T14:06:27Z
2016-11-23T14:06:27Z
2006
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/87240
 
Language en
 
Format application/pdf
 
Publisher CCSHAU