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Interspecific hybridization and plant regeneration in guar [Cyamopsis tetragonoloba (L.) Taub]

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Title Interspecific hybridization and plant regeneration in guar [Cyamopsis tetragonoloba (L.) Taub]
 
Creator S. Mathiyazhagan
 
Contributor Pahuja, S.K.
 
Subject Regeneration, Planting, Biological development, Developmental stages, Biological phenomena, Cytokinins, Hybridization, Crossing over, Pigeonpeas, Embryonic development
 
Description Guar (Cyamopsis tetragonoloba L.) is drought-tolerant multiple purpose cash
crop. Investigations were carried out an interspecific hybridization and to
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standardize medium for callus induction and plant regeneration. The crosses
between different genotypes of Cyamopsis tetragonoloba (female parent) and wild
species Cyamopsis serrata (male parent) were made. Out of 950 crosses few pods
developed with female parents namely, FS 277 and HVG 2-30 but no seed set was
found and pollen tube growth studies revealed presence of pre-fertilization
barriers. Different guar cultivars and the wild species of explants such as the
hypocotyls, cotyledon, and mature embryo were used for callus induction and these
explants along with cotyledonary node explants were used direct shoot
morphogenesis. It was established that Murashige and Skoog culture medium
containing 2,4-D ( 2.5 mg/l) and 6-benzylaminopurine (0.5 mg/l) gave the highest
frequency of callus induction using mature embryo explants in guar cultivars as
well as wild species. Over 2-3 shoots per explants were observed via somatic
embryogenesis on MS medium supplemented with NAA 2.0 mg/l+ BAP 0.5mg/l+
Charcoal 3g/l)) in cultivars HG 563 and FS 277 using mature embryo as explants.
Direct shoot regeneration was observed using cotyledonary node explants in guar
cultivars and its wild species on MS medium containing Kinetin (1.0 mg/l )+ BAP
0.5 (mg/l) and maximum 2-3of shoots per explants were observed on medium
supplemented with Kinetin (1.0 mg/l )+ BAP 0.5 (mg/l) and Zeatin (1.0mg/l ). No
shoot regeneration was observed in cotyledon, mature embryo, hypocotyl explants
of the guar cultivars and the wild species. In vitro rooting was observed on somatic
embryo derived shoots on a half-strength concentration of Murashige and Skoog’s
culture medium fortified with Indole-3-butyric acid (0.5 mg/l) + charcoal (3g/l).
 
Date 2016-11-16T11:40:03Z
2016-11-16T11:40:03Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85803
 
Language en
 
Format application/pdf
 
Publisher CCSHAU