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Isolation and characterization of plant growth promoting rhizobacteria from rhizosphere of shisham (Dalbergia sissoo)

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Title Isolation and characterization of plant growth promoting rhizobacteria from rhizosphere of shisham (Dalbergia sissoo)
 
Creator Padder, Shahid Ahmad
 
Contributor Pathak, D.V.
 
Subject Livestock, Genes, Biological phenomena, Proteins, Dna, Pcr, Productivity, Organic compounds, Polymorphism, Genetic structures
 
Description The hazardous nature of chemical fertilizers for the environment has led to a resurgence of
interest in the use of biofertilizer for enhanced environmental sustainability, lower cost production and
good crop yields. Numerous species of soil bacteria which flourish in the rhizosphere of plants have
been employed to ameliorate the fertility of soil & yield of the crops. To explore such microflora, a
total of 100 rhizospheric bacteria were isolated from the rhizosphere of Dalbergia sissoo, purified &
stained (Gram stained & spore stained). It was observed that 27% isolates were Gram positive, 73% of
the isolates were Gram negative & 66.66% Gram positive isolates were spore formeres. Finally
screening was done for advantageous traits viz. IAA production, ammonia secretion, phosphate
solubilization & biofungicidal activity. The effect of most Qutstanding isolates (sixteen in number) was
observed on the Dalbergia sissoo both under in vitro and pot house conditions. Then attempts were
made to identify these outstanding isolates upto genus level by studying morphological, physiological
& biochemical traits as per Bergey’s Manual of Deterministive Bacteriology. It was observed that 80%
of the isolated rhizospheric bacteria were IAA producers with the production ranging from 2.292 to
70.135 ig mU1, in the same way it was observed that 25% total isolates were phosphate solubilizers
with solubilization index varying from 1.25 to 2.89. Ammonia production was determined qualitatively
& it was found that 50% isolated rhizobacteria produced ammonia in which 9% were very good
producers, further it was observed that 45% of the isolates were antifungal in nature in which 3 1.11%
were antifungal against Fusarium oxysporum, 33.33% were antifungal against Ganoderma lucidium &
35% of the isolates were active against both the fungus. Under in vitro conditions highest germination
percentage enhancement was reported with the isolate SD87 causing an increment of 1 1.93%, root
growth enhancement was highest reported with the isolate SD99 causing an increment from 3 .53 cm in
control to 4.73 cm & shoot growth increment was highest reported with SD25 causing an increment
from 5.011cm in control to 7.40 cm. Under pot house conditions SD97, SD99 & SD6 caused highest
increment in nodule number, shoot length & root length respectively.
 
Date 2016-10-26T11:19:38Z
2016-10-26T11:19:38Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/82071
 
Language en
 
Format application/pdf
 
Publisher CCSHAU