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ORPHOLOGICAL AND MOLECULAR CHARACTERISATION OF CHRYSANTHEMUM (Chrysanthemum grandiflorum) GERMPLASM

KrishiKosh

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Title ORPHOLOGICAL AND MOLECULAR CHARACTERISATION OF CHRYSANTHEMUM (Chrysanthemum grandiflorum) GERMPLASM
 
Creator Gunjeet Kumar
 
Contributor Kanwar Pal Singh
 
Subject developmental stages, chrysanthemum, genetics, rapd, planting, fruits, genotypes, dna, polymorphism, biological phenomena
 
Description t-8169
Chrysanthemum (Chrysanthemum grandiflorum) has been bred for 3,000
years in China and Japan. It is one of the major cut flower crops in the domestic as
well as international trade. With the increasing demand of novelty large number of
cultivars are being bred every year in the world. Precise identification and
characterisation for establishing distinctness of cultivars becomes essential for
enforcing the intellectual property rights (IPRs) of breeders in the era of globalization.
Plant characterization provides description of the material and is being carried out
using morphological and molecular markers. Molecular markers detect more variation
and are not affected by environment. The morphological characterization was carried
out on 54 varieties of chrysanthemum using 76 descriptors as per UPOV guidelines for
DUS testing in Chrysanthemum and molecular characterization was done using 22
RAPD and 23 ISSR primers. The digital documentation of characterisation for nine
descriptors is presented. The detailed characterisation has been documented. The
varieties Texas Gold, Golden Yellow and Dolly Orange were the dwarfs and can be
used as pot mums or bedding purposes. The cultivar Tokyo Soldier produced
extremely big flowers. High heritability coupled with high genetic advance as percent
of mean were estimated for length of ray floret corolla tube, leaf thickness, length of
outer ray florets and peduncle length of terminal flower suggesting presence of
additive gene action for these traits. The higher estimates of SDI (>.50), indicating
greater diversity for that trait were recorded for predominant shape of leaf base,
longitudinal axis of ray florets of inner rows, height of flower head, pattern of second
color of inner side of ray florets, stem color and outer side color of ray floret where
markedly different to inner side.
The UPGMA dendogram pattern based on combined RAPD and ISSR analysis
showed that the two accessions of outlier species grouped separately and showed 72%
dissimilarity with other cultivars. The cultivars Maghi Orange, Maghi Yellow shared
the maximum similarity (97%) and were genetically close to cv Maghi White. The
other cultivars sharing higher similarity were Flirt with Yellow Gold and Pink Cloud
with Corcon Small. The estimates for number of cultivars identified by RAPD primers
ranged between 0 – 49 whereas for ISSR primers, it ranged between 0 - 47 (ISSR 31).
 
Date 2016-12-23T12:05:44Z
2016-12-23T12:05:44Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/92405
 
Format application/pdf