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Histomorphochemical and ultrastructural characterization of hypothalamo-hypophyseal-ovarian axis in Indian buffalo (Bubalus bubalis)

KrishiKosh

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Title Histomorphochemical and ultrastructural characterization of hypothalamo-hypophyseal-ovarian axis in Indian buffalo (Bubalus bubalis)
 
Creator Pathak, Devendra
 
Contributor Bansal, Neelam
 
Subject Buffalo
hypothalamus
hypophysis
histomorphochemical
Ultrastructure
Ovary
 
Description Present research work was conducted to study the histomorphochemical, histoenzymic, ultrastructural and immunohistochemical aspects of hypothalamo-hypophyseal-ovarian axis (HHO) of Indian buffalo during different stages of its reproductive cycle. Eight nuclei were distinctly recognized in buffalo hypothalamus as paraventricular, supraoptic, preoptic, arcuate, ventromedial, lateral mammillary, medial mammillary and posterior hypothalamic nucleus. Ultrastructurally, hypothalamic neurons had round nucleus, myelinated axons, different types of junctions and in between neurons astroglia and blood vessels with blood brain barrier. Vascular endothelial growth factor (VEGF) was localized in the neurons and endothelial cells of various nuclei and highest percentage positive cells were observed in follicular phase. Anterior pituitary gland had granular and agranular cells. Ultrastructurally, cells were categorized as somatotrophs, lactotrophs, gonadotrophs, corticotrophs, thyrotrophs and folliculostellate cells (FSC). FSC having cytoplasmic connections with granulocytes and were immunostained with Anti-Vimentin antibody and Anti-S-100 protein establishing its role as paracrine control and stem cell like actions. Anti ER and Anti PR antibodies were localized in pars distalis and pars intermedia and highest percentage positive cells were observed during the follicular phase. VEGF was localized in the endothelial cells of vessels as well as endocrine cells of pituitary and distinctly marked the portal vessels. Ovarian follicles and corpus luteum were characterized histomorphochemically, histoenzymatically, ultrastructurally. ER and PR were localized in different compartment of ovary and highest percentage positive cells were observed during the follicular phase. The VEGF was localized in the pre antral follicle, antral follicle and corpus luteum. Overall activity of the HHO axis was highest during the follicular phase of the estrous cycle indicating the estrogen hormone and its receptor are the key to maintain this axis in a coordinated manner.
 
Date 2016-08-25T11:15:23Z
2016-08-25T11:15:23Z
2015-03-25
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73729
 
Language en
 
Format application/pdf