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Phenotyping and molecular marker analysis of segregating population derived from interspecific hybrid in cluster bean [Cyamopsis tetragonoloba (L.) Taub.]

KrishiKosh

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Title Phenotyping and molecular marker analysis of segregating population derived from interspecific hybrid in cluster bean [Cyamopsis tetragonoloba (L.) Taub.]
 
Creator Sharma, Manisha
 
Contributor Yadav, Neelam R.
 
Subject Farmer field schools, Integrated pest management, Rice, Land resources, biological phenomena, Irrigation, Marketing, Productivity, Diseases, crops
 
Description Cluster bean (Cyamopsis tetragonoloba) is a drought tolerant, summer annual legume. Earlier,
the crop was used as forage and vegetable but now its galactomannan gum has gained the primary
importance making it an industrial crop. There is great demand of guar gum and its derivatives but the
production is less than the demand. Bacterial leaf blight (BLB) causes 60-80 per cent yield losses. So,
farmers are interested in disease resistant and high yielding varieties. In our lab, we developed the
interspecific hybrids by using HG563 (high gum content but susceptible to BLB) and C. serrata
(resistant to BLB but has low gum content). The segregating (F2-3) population was used in this study for
molecular marker analysis using 47 EST-SSRs which are related to disease resistance and
galactomannan metabolism. Eleven EST-SSRs were related to the disease resistance and 36 were
related to the galactomannan metabolism. These were used to study polymorphism in the parents and a
total of 18 (five related to disease resistance and 13 related to galactomannan metabolism) were found
to be polymorphic. These polymorphic EST-SSRs were used for screening of 91 plant of F2-3
population. Cluster analysis showed two major clusters formed at the similarity coefficient of 0.55. The
one of the major cluster was again differentiated into two sub-clusters at the similarity coefficient of
0.57. The cultivated species i.e. HG563 along with four plants of F2-3 population clustered in major
cluster I, while the wild species i.e. C. serrata along with three plants of F2-3 population clustered in
sub-cluster I of major cluster II, present at extreme ends of dendrogram. All other plants of F2-3
population were clustered in sub-cluster II in between of cluster I and sub-cluster I. Phenotypic analysis
of selected traits was carried out under field conditions and a great variation was observed for all the
selected attributes. A few promising plants based on phenotypic and molecular analysis has obtained.
 
Date 2016-10-18T11:19:25Z
2016-10-18T11:19:25Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/80831
 
Language en
 
Format application/pdf
 
Publisher CCSHAU