Record Details

POLLEN VIABILITY AND IN VITRO GERMINATION STUDIES IN ROSE

KrishiKosh

View Archive Info
 
 
Field Value
 
Title POLLEN VIABILITY AND IN VITRO GERMINATION STUDIES IN ROSE
 
Creator PRATIVA LAKHOTIA
 
Contributor D.V.S. Raju
 
Subject Service failed, response=Internal Server Error
 
Description T-8508
The present study was conducted to screen the male donor parents in rose for pollen efficiency and to find out the effect of season on pollen viability, in vitro pollen germination and hip set. To screen male donors, ten cultivars of rose viz., Africa Star, Century Two, Dr. Bharatram, Eiffel Tower, Folklore, Gold Medal, Kiss of Fire, Pinata, Pusa Ajay and Sadabahar were taken. Pollen viability, in vitro pollen germination, absolute pollen viability, pollen diameter and hip set were observed. Five treatments were used for in vitro pollen germination viz., Control (distilled water) (T1), 15% sucrose (T2), 20% sucrose (T3), 15% sucrose + 100 ppm boric acid (T4), 20% sucrose + 100 ppm boric acid (T5). The ten varieties were crossed to three female parents viz., Queen Elizabeth, Pink Parfait and Raktagandha for assessing hip set. Pollen viability varied from 6.45% (Folklore) to 78.07% (Africa Star) among the cultivars. The percentage pollen germination and absolute pollen viability was highest in Dr. Bharatram followed by Africa Star. The pollen of the cultivars Folklore and Pusa Ajay failed to germinate in all the five treatments, neither did they set hip. Higher hip set was recorded with pollen of cultivars Africa Star and Dr. Bharatram. The treatment T2 (15% sucrose) recorded the highest percent of pollen germination among all the five treatments. Pollen diameter was higher in cultivars Africa Star and Dr. Bharatram and lower in cultivars Folklore and Pusa Ajay. Pollen viability, in vitro pollen germination, absolute pollen viability and hip set showed positive correlation with pollen diameter. The cultivars Africa Star and Dr. Bharatram were found to be the best pollen donor among the varieties used for screening. Pollen viability percent and pollen diameter were found to be the best parameters for ascertaining the pollen fertility.
To find out the seasonal variation in pollen viability, in vitro pollen germination and hip set, pollen was collected in the month of December, January and February from four cultivars viz., Angelique, Pink Parfait, Pinata and Queen Elizabeth. To assess the hip set in the three months Pusa Ajay and Raktagandha were chosen as female parents. Pollen viability varied significantly among the three months with the highest pollen viability in the month of February. In vitro pollen germination and absolute pollen viability were higher in the month of January. February was the best month for attempting hybridization in rose as the maximum hip set was observed in this month. Pollen viability, hip set showed a positive correlation with temperature and relative humidity. Light duration had no significant influence on pollen viability, in vitro pollen germination and hip set.
 
Date 2016-12-01T09:31:32Z
2016-12-01T09:31:32Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/88498
 
Format application/pdf
 
Publisher IARI, DIVISION OF FLORICULTURE AND LANDSCAPING