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ANALYSIS OF INHERITANCE AND STABILITY OF TRANSGENE(S) (psy+crtI) AND DEVELOPMENT OF PROVITAMIN A RICH indica RICE LINE THROUGH MARKER ASSISTED BACKCROSS BREEDING

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Title ANALYSIS OF INHERITANCE AND STABILITY OF TRANSGENE(S) (psy+crtI) AND DEVELOPMENT OF PROVITAMIN A RICH indica RICE LINE THROUGH MARKER ASSISTED BACKCROSS BREEDING
 
Creator CHIKKAPPA G. KARJAGI
 
Contributor A. K. Singh
 
Subject transgenics, planting, rice, selection, vitamins, genes, backcrossing, genetics, genomes, segregation
 
Description t-8165
Rice is one of the major staple food crops and feeds more than half of the world's population.
Since, rice plant lacks the pathway to synthesize and accumulate provitamin A in the
endosperm, the rice eaters particularly children, and pregnant women suffer due to vitamin A
deficiency. The pathway for provitamin A synthesis in rice endosperm has now been
engineered through transgenesis and transgenic rice lines have yellow endosperm, hence
named as ‘Golden Rice’. The present study aimed at studying the inheritance of transgene(s)
in six transgenic events of ‘Golden Rice’ and transfer of provitamin A trait from transgenic
lines to a mega rice variety Swarna, which is widely grown in India. With respect to its first
objective of studying the inheritance and stability of transgene(s), the transgene inheritance
was studied in 16 different populations. The two events G1 and T1 out of the six events
studied showed distorted segregation in BC1F2 and BC2F2 respectively in Swarna genetic
background. The rest of the events (E1, R1, L1 and W1) followed the normal Mendelian
inheritance in BC1F1 and BC1F2 generations. The distorted segregation may be due to
differential transmission of transgene carrying gametes either from male or from female
parents. As part of the second objective of introgression of provitamin A trait into Swarna
genetic background, marker assisted backcross breeding approach was used for identifying
transgene positive plants in segregating populations through event specific primers
(foreground selection) and also for recovering plants with highest RPG content (background
selection). MABB was also effective in reducing the time to develop the NILs. In the present
study it was possible to recover plant with as high as 97% (BC2F2-19-32-64) recurrent parent
genome based on background selection using 67 polymorphic markers. The MAS derived
lines were analyzed for nine yield and yield contributing traits under containment. The
several NILs (BC2F2-19-32-25, BC2F2-19-32-43 etc.) were identified based on the agronomic
performances as comparable to recurrent parent Swarna. Finally the NILs were analyzed for
total cartenoid content and some of the lines were identified with as high as 16µg/g (BC2F2-
19-32-28) of endosperm. In general the provitamin A content varied from 4 to 16 microgram
per gram of rice endosperm. To make these lines to be exploited commercially there is need
for agronomical evaluation at large scale.
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Date 2016-12-23T11:46:10Z
2016-12-23T11:46:10Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/92396
 
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